马铃薯块茎不同发育时期淀粉合成相关基因表达与淀粉颗粒大小分析  

Analysis of Starch Synthesis Related Gene Expression and Starch Granule Size during Tuber Developmental Stages of Potato

作　　者：刘婕 艾菊 高冬丽 LIU Jie;AI Ju;GAO Dongli(Yunnan Key Laboratory of Potato Biology,Yunnan Normal University,Kunming 650500,China)

机构地区：[1]云南师范大学云南省马铃薯生物学重点实验室,云南昆明650500

出　　处：《云南师范大学学报（自然科学版）》2022年第5期32-36,共5页Journal of Yunnan Normal University:Natural Sciences Edition

基　　金：国家自然科学基金资助项目(32060684)。

摘　　要：淀粉的合成受一系列酶协同作用调控,明确块茎发育过程中淀粉合成相关基因的表达模式和淀粉颗粒粒径的变化规律,可以为马铃薯高淀粉育种提供分子基础.以块茎发育7个时期的样品为研究对象,用实时荧光定量PCR方法检测SuSy4等8个淀粉合成相关基因的表达量,用显微镜法测定淀粉颗粒粒径.结果表明,淀粉合成相关基因在块茎中高表达,在块茎发育时期,后期表达要高于前期表达.在发育过程中,小颗粒淀粉占比最大,大颗粒淀粉占比总体呈现出逐渐增加的趋势.Starch synthesis is coordinated by multiple enzymes.Analysis of starch synthesis related gene expression and starch granule size during developmental stages of potato tubers will provide a molecular basis for potato breeding aiming of high starch content.With the tuber samples of seven tuber developmental stages, we investigated the expression of eight starch synthesis related genes using quantitative reversetranscription PCR(RT-qPCR) method, and examined the granule size using microscopic method.The results showed that eight genes were preferentially expressed in tubers, and their transcript levels in the late stages were higher than that in the early stages of tuber development.The small starch granule with size < 20 μm made up of the majority starch granule, and the proportion of large granules with size ≥ 20 μm generally increased with the tuber development.

关 键 词：马铃薯 淀粉合成相关基因 表达 颗粒大小 

分 类 号：S532[农业科学—作物学]