产生物表面活性剂菌株的筛选及发酵条件优化  

Screening of biosurfactant-producing strains and optimization of fermentation conditions

作　　者：董丁 姜波 杨行 季凯 余何美哲 DONG Ding;JIANG Bo;YANG Hang;JI Kai;YUHE Mei-zhe(Karamay Xin'aoda Petroleum Technology Service Co.,Ltd,Karamay 834000,China)

机构地区：[1]克拉玛依市新奥达石油技术服务有限公司,新疆克拉玛依834000

出　　处：《生物技术》2022年第4期500-505,共6页Biotechnology

基　　金：新疆维吾尔自治区科技支疆项目(2019E0204)。

摘　　要：[目的]筛选高产表面活性剂的菌株,并探究合适的培养条件。[方法]以被石油类物质污染的土壤为样品,通过排油圈和表面张力实验筛选出生物表面活性剂高产菌,结合单因素优化得出最佳发酵条件。[结果]通过初筛和复筛最终得到一株表面活性剂高产菌株FC-69,经16S rDNA鉴定为铜绿假单胞菌(Pseudomonas aeruginosa)。最终得出以20 g/L葡萄糖,3 g/L硝酸铵,NaCl 5 g/L,初始pH值8.0,培养温度30℃的培养条件更有利于FC-69菌株生物表面活性剂的合成。[结论]成功筛选出一株高产生物表面活性剂的菌种,结合优化后的发酵条件可使筛选菌株的生物表面活性剂产量达到31.61 g/L。[Objective]In order to screen the strains with high surfactant yield and explore the suitable culture conditions.[Method]Taking the soil polluted by petroleum as a sample,the biosurfactant-producing bacteria were screened out by oil drainage circle and surface tension experiment,and the optimal fermentation conditions were obtained by single factor optimization.[Result]A high-yield surfactant strain FC-69 was finally obtained through primary screening and secondary screening.After 16S rDNA identification,the obtained strain was Pseudomonas aeruginosa.Finally,it is concluded that the culture conditions of 20 g/L glucose,3 g/L ammonium nitrate,5 g/L salt,8.0 initial pH value of culture medium and 30℃culture temperature are more favorable for the synthesis of biosurfactant of FC-69 strain.[Conclusion]A strain with high biosurfactant yield was successfully screened.Combined with the optimized fermentation conditions,the biosurfactant yield of the screened strain could reach 31.61 g/L.

关 键 词：生物表面活性剂 分离 表面张力 发酵条件 优化 

分 类 号：Q939.97[生物学—微生物学]