丹参凝胶治疗对特应性皮炎小鼠模型皮肤屏障功能、表皮增生以及免疫功能的影响  

Effects of Salvia Gel Treatment on Skin Barrier Function,Epidermal Hyperplasia and Immune Function in Atopic Dermatitis-like Mouse Model

作　　者：郭玉冰 王潇 王芯[3] 李星[3] 李颖慧 GUO Yu-bing;WANG Xiao;WANG Xin;LI Xing;LI Ying-hui(Department of Dermatology and Venereal Diseases,The First Affiliated Hospital of Xian Jiaotong University,Xian,Shaanxi,71089,China;Department of Dermatology,The Red Cross Hospital Affiliated to Xi'an Jiaotong University,Xian,Shaanxi,710o00,China;Department of Dermatology and Venereal Diseases,Second Affiliated Hospital of Xian Jiaotong University,Xi'an,Shaanxi,710049,China;Department of Dermatology,Xjing Hospital,Xian,Shaanxi,710032,China)

机构地区：[1]西安交通大学第一附属医院皮肤性病,科陕西西安710089 [2]西安交通大学附属红会医院皮肤科,陕西西安710000 [3]西安交通大学第二附属医院皮肤性病科,陕西西安710049 [4]西京医院皮肤科,陕西西安710032

出　　处：《现代生物医学进展》2022年第17期3220-3224,共5页Progress in Modern Biomedicine

基　　金：陕西省自然科学基础研究计划项目(2021JM-572)。

摘　　要：目的:研究丹参凝胶治疗对特应性皮炎小鼠模型皮肤屏障功能、表皮增生以及免疫功能的影响。方法:30只C57BL/6小鼠被随机分为Control组、AD组和SG组,每组10只。AD组和SG组背部涂抹对二硝基氟苯建立特应性皮炎小鼠模型,SG小鼠在模型建立成功后涂抹丹参凝胶治疗3周,Control组和AD组涂抹凡士林作为对照。3周后,测量所有小鼠经皮水分丢失量(TEWL)、皮肤厚度、脾脏指数、胸腺指数,血清Ig E、IFN-γ和IL-4,脾脏树突状细胞、Th1和Th2细胞比例。结果:丹参凝胶治疗3周后,AD组和SG组小鼠TEWL、皮肤厚度、脾脏指数、胸腺指数,血清Ig E、IFN-γ和IL-4含量,以及脾脏Th2细胞比例均显著高于对照组正常小鼠(P<0.05),而脾脏树突状细胞、Th1细胞和Th1/Th2细胞比例均显著低于对照组正常小鼠(P<0.05);与AD组小鼠相比,SG组小鼠TEWL、皮肤厚度、脾脏指数、胸腺指数,血清Ig E、IFN-γ和IL-4含量,以及脾脏Th2细胞比例均显著降低(P<0.05),而脾脏树突状细胞、Th1细胞和Th1/Th2细胞比例均显著升高(P<0.05)。结论:丹参凝胶具有保护特应性皮炎样小鼠皮肤屏障功能和抑制表皮增生的功能,并且可以影响特应性皮炎样小鼠脾脏树突状细胞和辅助性T细胞比例。Objective:To study the effect of Salvia gel treatment on skin barrier function,epidermal hyperplasia and immune function in atopic dermatitis mouse model.Methods:Thirty C57BL/6 mice were randomly divided into Control group,AD group and SG group,10 mice in each group.AD group and SG group were smeared with p-dinitrofluorobenzene on the back to establish atopic dermatitis mouse model,SG mice were smeared with Salvia gel for 3 weeks after the model was successfully established,and the control group and AD group were smeared with vaseline as a control.After 3 weeks,the transepidermal water loss(TEWL),skin thickness,spleen index,thymus index,serum Ig E,IFN-γand IL-4,spleen dendritic cells,Th1 and Th2 cell ratios were measured in all mice.Results:After 3 weeks of Danshen gel treatment,the TEWL,skin thickness,spleen index,thymus index,serum Ig E,IFN-γand IL-4contents,and the proportion of spleen Th2 cells in AD group and SG group were significantly higher than those in the control group.mice(P<0.05),while the proportions of dendritic cells,Th1 cells and Th1/Th2 cells in the spleen were significantly lower than those of normal mice in the control group(P<0.05);compared with the AD group mice,the SG group mice TEWL,skin thickness,spleen index,thymus index,serum Ig E,IFN-γand IL-4 levels,and the proportion of spleen Th2 cells were significantly decreased(P<0.05),while spleen dendritic cells,Th1 cells and Th1/Th2 The proportion of cells was significantly increased(P<0.05).Conclusion:Salvia gel can protect the skin barrier function of atopic dermatitis-like mice and inhibit epidermal hyperplasia,and can affect the ratio of dendritic cells and helper T cells in the spleen of atopic dermatitis-like mice.

关 键 词：丹参凝胶 特发性皮炎 皮肤屏障 表皮增生 免疫功能 

分 类 号：R-33[医药卫生] R751