简青霉CEF-818固体发酵工艺优化  

Optimization of Solid-State Fermentation Process of Penicillium simplicissimum CEF-818

作　　者：蒲丹丹 李艳 赵丽红 张亚林 魏锋 冯鸿杰[2] 朱荷琴[2] 顾爱星[1] 彭军 冯自力[2] PU Dandan;LI Yan;ZHAO Lihong;ZHANG Yalin;WEI Feng;FENG Hongjie;ZHU Heqin;GU Aixing;PENG Jun;FENG Zili(Engineering Research Centre of Cotton,Ministry of Education/College of Agriculture,Xinjiang Agricultural University,Urumqi 830052,China;State Key Laboratory of Cotton Biology/Institute of Cotton Research of Chinese Academy of Agricultural Sciences,Anyang 455000,China)

机构地区：[1]新疆农业大学农学院/棉花教育部工程研究中心,乌鲁木齐830052 [2]中国农业科学院棉花研究所/棉花生物学国家重点实验室,安阳455000

出　　处：《中国生物防治学报》2022年第3期636-644,共9页Chinese Journal of Biological Control

基　　金：国家棉花产业技术体系(CARS-15-22);棉花生物学国家重点实验室自主课题(CB2021C12);中国农业科学院科技创新工程(CAAS-ASTIPCCRI)。

摘　　要：简青霉Penicillium simplicissimum CEF-818发酵产物能够有效的防治由大丽轮枝菌Verticillium dahliae引起的棉花黄萎病,为获得简青霉CEF-818最佳的发酵条件,本研究采用单因素和正交试验方法对简青霉CEF-818固体发酵培养基的组成以及发酵条件进行了优化。结果表明,简青霉CEF-818固体发酵最适培养物为麦麸,其他添加物为葡萄糖、尿素和氯化钾,添加量按麦麸的质量比分别为2.5%、0.6%和0.6%(M/M),料水比为1:0.5,固体发酵最佳的培养条件为液体接种量为5%(V/M)、初始pH为7、物料厚度为2 cm、培养温度为28℃、培养时间为7 d。在此条件下,简青霉CEF-818的产孢量为8.28×10^(9) CFU/g。The fermentation product of Penicillium simplicissimum CEF-818 can effectively control cotton Verticillium wilt caused by Verticillium dahliae.In order to optimize fermentation conditions of P.simplicissimum CEF-818,the composition of solid fermentation medium and fermentation conditions of CEF-818 were screened by single factor and orthogonal test.The results showed that the optimum culture for solid fermentation of P.simplicissimum CEF-818 was wheat bran,and addition of 2.5%glucose (M/M),0.6%urea (M/M) and 0.6%potassium chloride (M/M),with the initial pH value of 7.0,the ratio of material to water 1:0.5,the liquid inoculums level of 5%(V/M),the material thickness of 2 cm and at 28℃for 7 days.Under this optimal medium and fermentation process,the sporulation could reach to 8.28×10^(9) CFU/g for P.simplicissimum CEF-818.

关 键 词：生物防治 简青霉 固体发酵 产孢量 

分 类 号：S476[农业科学—农业昆虫与害虫防治]