不同龄期草地贪夜蛾对常用杀虫剂的敏感性  

Susceptibility of Different Instar Larvae of Spodoptera frugiperda to Commonly Used Insecticides

作　　者：陈金翠[1] 曹利军[1] 马中正 苑新新 宫亚军[1] 沈修婧 魏书军[1] CHEN Jincui;CAO Lijun;MA Zhongzheng;YUAN Xinxin;GONG Yajun;SHEN Xiujing;WEI Shujun(Institute of Plant Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;Laiyuan Vocational and Technical Education Center,Laiyuan 074399,China)

机构地区：[1]北京市农林科学院植物保护环境保护研究所,北京海淀100097 [2]涞源县职业技术教育中心,河北涞源074399

出　　处：《广东农业科学》2022年第8期81-86,共6页Guangdong Agricultural Sciences

基　　金：北京市农林科学院创新能力建设专项(KJCX20200432)。

摘　　要：【目的】明确草地贪夜蛾(Spodoptera frugiperda)不同龄期幼虫对常用杀虫剂的敏感性差异,为该虫化学防治提供科学依据。【方法】在室内测定10种杀虫剂对草地贪夜蛾三龄幼虫的防效,通过生物测定比较一龄、三龄、五龄幼虫对防效高于85%的4种药剂的敏感性。【结果】氯虫苯甲酰胺、甲氨基阿维菌素苯甲酸盐、虫螨腈对草地贪夜蛾三龄幼虫药后7 d的防效均为100%,虱螨脲的防效为91.67%,溴氰虫酰胺的防效为88.89%,其余5种杀虫剂的防效较低,在44.44%~69.44%之间。生物测定结果表明,甲氨基阿维菌素苯甲酸盐药后48 h对一龄、三龄、五龄幼虫的致死中浓度(LC50)分别为0.0190、0.0234、0.3918 mg/L,氯虫苯甲酰胺药后72 h的LC50分别为0.2167、0.2763、3.3718 mg/L,虫螨腈为1.1286、3.0194、19.2726 mg/L,虱螨脲为1.2213、2.1445、7.8419 mg/L。可见,随着龄期的增加,草地贪夜蛾幼虫对4种药剂的敏感性逐渐降低,五龄幼虫的敏感性显著低于一龄和三龄幼虫。【结论】草地贪夜蛾幼虫为1~3龄时推荐使用氯虫苯甲酰胺、甲氨基阿维菌素苯甲酸盐、虫螨腈和虱螨脲进行防治,当幼虫发育至五龄时推荐使用甲氨基阿维菌素苯甲酸盐或氯虫苯甲酰胺。【Objective】The study aims to understand the susceptibility variation of different instar larvae of Spodoptera frugiperda to commonly used insecticides,with a view to providing scientific basis for chemical control of this pest.【Method】The control efficacy of 10 insecticides to the larvae of the S.frugiperda was determined in the laboratory.The susceptibility of the first,third and fifth instar larvae to four insecticides with the control efficacy of over 85% was compared by the bioassay method.【Result】The control efficacy of chlorantraniliprole,emamectin benzoate and chlorfenapyr to the third instar larvae of S.frugiperda was 100% seven days after treatment,while that of lufenuron was 91.67%,and that of cyantraniliprole was 88.89%.The remaining five insecticides had a low control efficacy,ranging from 44.44% to 69.44%.Bioassay results showed that the LC50 values of emamectin benzoate to the first,third and fifth instar larvae were 0.019,0.0234,and 0.3918 mg/L respectively 48 hours after treatment.The LC50 of chlorantraniliprole to the first,third and fifth instar larvae were 0.2167,0.2763,and 3.3718 mg/L 72 hours respectively after treatment,,while that of chlorfenapyr was 1.1286,3.0194 and 19.2726 mg/L,and that of lufenuron was 1.2213,2.1445 and 7.8419 mg/L.It could be seen that the susceptibility of S.frugiperda to insecticides was decreased with the increase of instar.The fifth instar larvae of S.frugiperda had significantly lower susceptibility to the four tested insecticides than the first and third instar larvae.【Conclusion】The chlorantraniliprole,emamectin benzoate,chlorfenapyr and lufenuron were recommended for the control of first to third instar larvae of S.frugiperda.The emamectin benzoate and chlorantraniliprole were recommended to control fifth instar larvae.

关 键 词：草地贪夜蛾 幼虫 龄期 杀虫剂 防治效果 生物测定 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治] S482.3[农业科学—植物保护]