甲状腺乳头状癌组织miR-144-5p、FN1表达变化及其与临床病理特征和预后的关系  

Expression changes of miR-144-5p and FN1 in papillary thyroid cancer tissues and their relationships with clinicopathological features and prognosis

作　　者：李卫[1] 王猛[1] 刘杰 LI Wei;WANG Meng;LIU Jie(Head and Neck Thyroid Surgery,Cangzhou Hospital of Integrated TCM-WM,Cangzhou 061000,China)

机构地区：[1]河北省沧州中西医结合医院头颈甲状腺外科,河北沧州061000

出　　处：《山东医药》2022年第25期25-29,共5页Shandong Medical Journal

基　　金：河北省医学科学研究课题计划(20210957)。

摘　　要：目的探讨甲状腺乳头状癌(PTC)组织微小RNA-144-5p(miR-144-5p)、纤连蛋白1(FN1)表达变化及其与临床病理特征和预后的关系。方法选择PTC患者78例,取术中切除的PTC组织及其配对的癌旁正常组织,采用RT-qPCR法检测miR-144-5p、FN1表达。比较PTC组织与癌旁正常组织miR-144-5p、FN1表达,并分析PTC组织miR-144-5p表达与FN1表达的关系以及二者表达与临床病理特征的关系。术后随访3年,统计3年总生存率。分别以miR-144-5p、FN1相对表达量的均值为界,将PTC患者分为miR-144-5p高表达者与低表达者、FN1高表达者与低表达者,分析PTC组织不同miR-144-5p、FN1表达与患者3年总生存率的关系。采用多因素Cox回归模型分析PTC患者预后不良的影响因素。结果与癌旁正常组织比较,PTC组织miR-144-5p表达显著降低、FN1表达显著升高(P均<0.05)。Spearman相关分析显示,PTC组织miR-144-5p表达与FN1表达呈负相关关系(r=-0.772,P<0.01)。PTC组织miR-144-5p、FN1表达与TNM分期、淋巴结转移有关(P均<0.05),而与性别、年龄、肿瘤直径、多灶性、侵及被膜无关(P均>0.05)。随访4~36个月,中位随访27个月,随访期间死亡14例,术后3年总生存率为82.05%(64/78)。miR-144-5p高表达者39例、低表达者39例,FN1高表达者40例、低表达者38例。生存分析显示,miR-144-5p高表达者术后3年总生存率高于其低表达者,FN1低表达者术后3年总生存率高于其高表达者(P均<0.05)。多因素Cox回归分析显示,TNM分期Ⅲ、Ⅳ期及有淋巴结转移、FN1表达≥15.29为PTC患者预后不良的独立危险因素,而miR-144-5p表达≥0.67则为其独立保护因素(P均<0.05)。结论PTC组织miR-144-5p低表达、FN1高表达,二者表达与肿瘤TNM分期、淋巴结转移和患者预后密切相关。Objective To observe the expression changes of microRNA-144-5p(miR-144-5p)and fibronectin 1(FN1)in the papillary thyroid carcinoma(PTC)tissues and their relationship with clinicopathological features and prognosis.Methods Seventy-eight cases of patients with PTC were selected.The PTC tissues removed during the operation and their matched normal tissues adjacent to the cancer were taken.The miR-144-5p and FN1 expression levels were detected by RT-qPCR.The expression of miR-144-5p and FN1 in PTC tissues was compared with that in adjacent normal tissues,and the relationship between the expression of miR-144-5p and FN1 in PTC tissue was analyzed and the relationships between their expression and the clinicopathological features of patients were analyzed.The patients were followed up for three years,and the overall survival rate was recorded.According to the mean value of the relative expression of miR-144-5p and FN1,PTC patients were divided into patients with high and low expression of miR-144-5p,and patients with high and low expression of FN1.The relationship between the expression of miR-144-5p and FN1 in PTC tissues and the 3-year overall survival rate of patients were analyzed.Multivariate Cox regression model was used to analyze the influencing factors for poor prognosis in PTC patients.Results Compared with the normal tissues adjacent to the cancer,the miR-144-5p expression decreased and FN1 expression increased in PTC tissues(both P<0.05).Spearman correlation analysis showed that miR-144-5p was negatively correlated with FN1 expression in PTC tissues(r=-0.772,P<0.01).The miR-144-5p and FN1 expression levels were related to TNM stage and lymph node metastasis in PTC patients(all P<0.05),but not to sex,age,tumor diameter,multifocality,or invasion of capsule(all P>0.05).The follow-up period ranged from 4 to 36 months,with a median of 27 months,14 cases of patients died during the follow-up period.The 3-year overall survival rate was 82.05%(64/78).There were 39 cases of high expression of miR-144-5p,39 cases of

关 键 词：甲状腺乳头状癌 微小RNA-144-5p 纤连蛋白1 临床病理特征 预后 

分 类 号：R736.1[医药卫生—肿瘤]