Putrescine Enhances Seed Germination Tolerance to Heat Stress in Arabidopsis thaliana  

作　　者：Shiyan Lu Yulan Hu Yilin Chen Yaru Yang Yue Jin Ping Li Xiangyang Hu 

机构地区：[1]Shanghai Key Laboratory of Bio-Energy Crops,Research Center for Natural Products,Plant Science Center,School of Life Sciences,Shanghai University,Shanghai,200444,China

出　　处：《Phyton-International Journal of Experimental Botany》2022年第9期1879-1891,共13页国际实验植物学杂志（英文）

基　　金：the National Natural Science Foundation of China(Grant No.32170562).

摘　　要：Putrescine(Put)as the compound of plant polyamines is catalyzed by arginine decarboxylase(ADC),which is encoded by two members,ADC1 and ADC2 in Arabidopsis,and ADC2 is mainly responsible for Put biosynthesis.Accumulated evidence demonstrates the important function of Put in plant growth and development,but its role in regulating seed germination under high temperature(HT)has not been reported yet.SOMNUS(SOM)is the negative regulator for seed germination thermoinhibition by altering downstream gibberellin(GA)and abscisic acid(ABA)metabolism.In this study,we found exogenous application of Put obviously alleviated the inhibition effect of HT on seed germination.Whereas pharmacological inhibition of endogenous Put level reduced seed germination under HT.Consistently,HT induced the rapid accumulation of Put level,and the adc2 mutant defi-ciency in Put biosynthesis also showed more sensitivity to HT stress.Furthermore,we found that the Put signal suppressed the expression of SOM and changed the transcriptional patterns of genes associated with GA/ABA metabolism.Genetic analysis also revealed SOM was epistatic to ADC2 to alter GA/ABA metabolism.Collectively,our finding reveals the novel function of Put in controlling seed germination under HT through SOM,and provides the possibility to develop Put as the innovational regulator for uniform seed germination under HT stress.

关 键 词：PUTRESCINE seed germination heat stress SOM ADC2 

分 类 号：Q94[生物学—植物学]