Combination of 6-Benzylaminopurine and Thidiazuron Promotes Highly Efficient Shoot Regeneration from Cotyledonary Node of Mature Peanut (Arachis hypogaea L.) Cultivars  

作　　者：Abraham Lamboro Xiao Han Songnan Yang Xueying Li Dan Yao Baixing Song Jun Zhang 

机构地区：[1]Department of Crop Genetics and Breeding,College of Agronomy,Jilin Agricultural University,Changchun,130118,China [2]Department of Biochemistry and Molecular Biology,College of Life Science,Jilin Agricultural University,Changchun,130118,China

出　　处：《Phyton-International Journal of Experimental Botany》2022年第12期2619-2631,共13页国际实验植物学杂志（英文）

基　　金：Jilin Province’s Key Research and Development Project(20180201070NY)for financial support.

摘　　要：Efficient in vitro plantlet regeneration is an important step to successfully transform genes for the improvement of agronomic traits.A combination of 6-benzylaminopurine(BAP)and thidiazuron(TDZ)plant growth regulators was applied to evaluate shoot regeneration capacity whereasα-naphthalene acetic acid(NAA)combination with 6-benzylaminopurine(BAP),and 2,4-dichlorophenoxyacetic acid(2,4-D)with 6-benzylaminopurine were tested to optimize root induction for two peanut cultivars.The result showed combination(BAP with TDZ)was found to be effective in promoting shoot.The highest shoot regeneration frequency(93%)was obtained on a medium supplemented with 4 mg/L BAP and 0.5 mg/L TDZ while an average regeneration frequency(87%)was achieved in a medium containing combinations of 2 mg/L BAP with 1 mg/L TDZ.The shooting rate increased for both cultivars as the concentrations of BAP increased and TDZ decreased.The highest rooting rate(93%)was obtained on a medium supplemented with 3.5 mg/L NAA with 2.5 mg/L BAP for both cultivars.The rooting rate increased as the concentration of auxin to cytokinin ratio increased.The maximum rooting rate(83%)was obtained on MS medium supplemented with 0.3 mg/L 2,4-D with 0.2 mg/L BAP for the cultivar N3.The result indicated that BAP with NAA was much better than BAP with 2,4-D in rooting rate.Thus,the protocol developed was genotype independent and effective for peanut tissue culture.

关 键 词：AUXIN cotyledonary node CYTOKININS in vitro PEANUT 

分 类 号：S565.2[农业科学—作物学]