宁夏枸杞根际土壤链霉菌V-1-3次级代谢产物分析  

Study on secondary metabolites of Streptomyces sp.V-1-3 from the rhizosphere soil of Lycium barbarum in Ningxia

作　　者：胡运琪 马晓莉 贾荣亮 林文星 郭欢欢 李彬 李舂龙[1] 南泽东 吴秀丽[3] 江志波 Hu Yun-qi;Ma Xiao-li;Jia Rong-liang;Lin Wen-xing;Guo Huan-huan;Li Bin;Li Chong-long;Nan Ze-dong;Wu Xiu-li;Jiang Zhi-bo(Key Laboratory of Chemical Engineering and Technology of State Ethnic Affairs Commission,School of Chemistry and Chemical Engineering,North Minzu University,Yinchuan 750021;Northwest Institute of Eco-Environment and Resources,Chinese Academy of Sciences,Lanzhou 730000;College of Pharmacy,Ningxia Medical University,Yinchuan 750004;Laboratory Construction and Management Office,North Minzu University,Yinchuan 750021)

机构地区：[1]北方民族大学化学与化学工程学院化工技术基础国家民委重点实验室,银川750021 [2]中国科学院西北生态环境资源研究院甘肃省寒区旱区逆境生理与生态重点实验室,兰州730000 [3]宁夏医科大学药学院,银川750004 [4]北方民族大学实验室建设与管理处,银川750021

出　　处：《中国抗生素杂志》2022年第7期647-653,共7页Chinese Journal of Antibiotics

基　　金：国家自然科学基金地区项目(No.82160672);北方民族大学研究生创新项目(No.YCX22156);四川省中医药管理局科技专项(No.2020JC0069)。

摘　　要：目的获得链霉菌V-1-3的基因组序列信息,分析其次级代谢产物生物合成基因簇并预测其代谢产物,为发现潜在新抗生素奠定基础。方法基于16S rRNA基因序列进行菌株属水平鉴定,利用Illumina HiSeq+PacBio测序技术对菌株V-1-3进行基因组测序,采用antiSMASH(v6.0.1)在线工具分析次级代谢产物生物合成基因簇,液-质联用技术检测产生的次级代谢产物。结果链霉菌V-1-3基因组序列全长8243417 bp,平均(G+C)含量为72.14%,共编码7578个基因,预测到33个生物合成基因簇,利用液-质联用检测到4个代谢物:oxalomycin B、geosmin、coelichelin和ishigamide。结论来自盐碱地的链霉菌V-1-3具有丰富的次级代谢产物生物合成基因簇,能产生多种次级代谢产物,具有进一步发掘新抗生素的价值。Objective To mine secondary metabolite biosynthesis gene clusters of Streptomyces sp.V-1-3,predict its metabolites and lay the foundation for the discovery of new antibiotics.Methods The 16S rRNA gene sequencing was employed to preliminarily identify the strain.Streptomyces sp.V-1-3 was sequenced by Illumina HiSeq+PacBio sequencing technology to mine secondary metabolite by antiSMASH(v6.0.1)online tool and the secondary metabolites of Streptomyces sp.V-1-3 were further analyzed by HPLC-Q-TOF MS.Results The size of the genome was 8,243417 bp with an average of G+C content at 72.14%,which had 7,578 genes.Biosynthetic gene clusters for thirty-three secondary metabolites were predicted.Four compounds,oxalomycin B,geosmin,coelichelin,and ishigamide were validated by HPLC-Q-TOF MS analysis.Conclusion Streptomyces sp.V-1-3 from salinealkali soil has abundant secondary metabolite biosynthesis gene clusters,and can produce different types of secondary metabolites,which has the value for further exploring to find new antibiotics.

关 键 词：盐碱地 链霉菌 全基因组测序 次级代谢产物 

分 类 号：R978.1[医药卫生—药品] Q939.1[医药卫生—药学]