大肠癌组织棕榈酰化修饰蛋白的组学分析  

Proteomic Analysis of Palmitoylated Proteins in Human Colon Carcinoma Tissues

作　　者：杨静波[1] 何成彦[2] 卢日峰[3] 刘宁[1] YANG Jing-Bo;HE Cheng-Yan;LU Ri-Feng;LIU Ning(Central Laboratory,the Second Hospital of Jilin University,Changchun 130041,China;Department of Clinical Laboratory,China-Japan Union Hospital of Jilin University,Changchun 130033,China;College of Public Health,Jilin University,Changchun 130012,China)

机构地区：[1]吉林大学第二医院医学研究中心,长春130041 [2]吉林大学中日联谊医院检验科,长春130033 [3]吉林大学公共卫生学院,长春130012

出　　处：《分析化学》2022年第10期1465-1472,共8页Chinese Journal of Analytical Chemistry

基　　金：吉林省科技厅项目(Nos.20210204103YY,20180101267JC)资助。

摘　　要：蛋白质棕榈酰化修饰在大肠癌的发生、发展和转移等过程中发挥了重要作用。本研究采用改进的酰基-生物素交换方法(Acyl-biotinyl exchange, ABE),对大肠癌组织中的棕榈酰化修饰蛋白进行富集,并利用蛋白质组学技术对富集的蛋白进行分析鉴定。结果共鉴定出213种高可信度(+HA/-HA>3)的棕榈酰化修饰蛋白,包括烯醇化酶1、过氧化物氧化还原酶、波形蛋白、线粒体苹果酸脱氢酶、核异质性核糖核蛋白K、分子伴侣复合体蛋白1等,并以烯醇化酶1棕榈酰化修饰为例,采用蛋白质印迹技术对其进行了验证。本研究对经典的ABE方法进行了改进,降低了非特异性背景信号,大肠癌癌组织中的棕榈酰化修饰蛋白的鉴定分析结果可为进一步研究特定肿瘤相关蛋白分子的棕榈酰化修饰调控的分子机制、精准发现大肠癌肿瘤标志物或分子靶点提供新思路。Protein palmitoylation plays important roles in the occurrence, development and metastasis of colon cancer. In this study, acyl biotinyl exchange(ABE) method was improved and used to enrich palmitoylated proteins in colorectal cancer tissues. The enriched proteins were identified by proteomics approaches. A total of 213 highly reliable palmitoylated proteins(+HA/-HA>3)were identified, including enolase 1, peroxide oxidoreductase, vimentin, mitochondrial malate dehydrogenase, nuclear heterogeneous ribonucleoprotein K, and chaperone complex protein 1. The palmitoylation modification of enolase 1 was verified by Western blot analysis. The present study improved the classical ABE method to reduce its non-specific background. The detection results of palmitoylated proteins in colon cancer tissues could provide a new idea for further studying the molecular mechanism of palmitoylation of specific tumor related proteins and accurately discovering tumor markers or molecular targets of colon cancer.

关 键 词：棕榈酰化修饰 大肠癌 蛋白质组 酰基-生物素交换 烯醇化酶1 

分 类 号：R735.34[医药卫生—肿瘤]