miR-5011-5p对膀胱癌细胞系J82凋亡和迁移的影响及机制研究  

Effects of miR-5011-5p on apoptosis and migration of bladder cancer cell line J82 and the underlying mechanism

作　　者：王蕾[1] 黄耿[2] 叶志华 姜卫东[2] 柯霓 Wang Lei;Huang Geng;Ye Zhihua;Jiang Weidong;Ke Ni(Department of Ultrasound Imaging,Huangshi Central Hospital of Edong Medical Group(Affiliated Hospital of Hubei Polytechnic University),Huangshi 435000,Hubei Province,China;Department of Urology,Huangshi Central Hospital of Edong Medical Group(Affiliated Hospital of Hubei Polytechnic University),Huangshi 435000,Hubei Province,China)

机构地区：[1]鄂东医疗集团黄石市中心医院,湖北理工学院附属医院超声影像科,黄石435000 [2]鄂东医疗集团黄石市中心医院,湖北理工学院附属医院泌尿外科,黄石435000

出　　处：《中国基层医药》2022年第9期1345-1348,共4页Chinese Journal of Primary Medicine and Pharmacy

基　　金：湖北省卫生健康委员会科研基金资助项目(WJ2019H158)。

摘　　要：目的观察miR-5011-5p对膀胱癌细胞系J82凋亡和迁移的影响并探讨可能的作用机制。方法以J82细胞为研究对象,分别转染随机序列分子(NC组)和miR-5011-5p序列分子(miR-5011-5p组)。通过流式细胞术和划痕实验分析miR-5011-5p对J82细胞凋亡和迁移的影响,通过生物信息学预测miR-5011-5p的靶基因,通过实时荧光定量聚合酶链式反应(RT-qPCR)和Western blot分别检测miR-5011-5p对靶基因表达的影响。结果miR-5011-5p组J82细胞中miR-5011-5p相对表达量为10.73±1.67,显著高于NC组的1.04±0.16(t=5.81,P<0.01)。NC组、miR-5011-5p组J82细胞凋亡率分别为(8.83±1.67)%、(34.96±3.80)%,差异有统计学意义(t=6.30,P<0.01)。NC组、miR-5011-5p组J82细胞迁移率分别为(71.31±7.69)%、(37.43±5.01)%,差异有统计学意义(t=3.69,P<0.05)。miR-5011-5p的靶基因可能是Yes相关蛋白1(YAP1)。与NC组相比,miR-5011-5p对J82细胞YAP1表达具有显著抑制效应(P<0.01)。结论miR-5011-5p可能通过靶向抑制YAP1基因表达,促进膀胱癌J82细胞的凋亡并抑制其迁移。Objective To investigate the effects of miR-5011-5p on apoptosis and migration of bladder cancer cell line J82 and the underlying mechanism.Methods J82 cells were transfected with random sequence molecules(NC group)and miR-5011-5p sequence molecules(miR-5011-5p group).Flow cytometry and scratch experiment were performed to analyze the effects of miR-5011-5p on apoptosis and migration of J82 cells.The target gene of miR-5011-5p was predicted by bioinformatics.Real-time fluorescent quantitative polymerase chain reaction and western blot assay were performed to investigate the effects of miR-5011-5p on target gene expression.Results The relative expression of miR-5011-5p in J82 cells in the miR-5011-5p group was significantly higher than that in the NC group(10.73±1.67 vs.1.04±0.16,t=5.81,P<0.01).There was significant difference in the apoptosis rate of J82 cells between NC and miR-5011-5p groups[(8.83±1.67)%vs.(34.96±3.80)%,t=6.30,P<0.01].The migration rate of J82 cells differed significantly between NC and miR-5011-5p groups[(71.31±7.69)%vs.(37.43±5.01)%,t=3.69,P<0.05].The target gene of miR-5011-5p may be Yes-related protein 1(YAP1).Compared with the NC group,miR-5011-5p exhibited an obvious inhibitory effect on the YAP1 expression in J82 cells(P<0.01).Conclusion miR-5011-5p may promote the apoptosis of J82 cells and inhibit their migration in bladder cancer through targeted inhibition of YAP1 gene expression.

关 键 词：膀胱肿瘤 核糖核酸酶类 非编码RNA 微小RNA 肿瘤转移 细胞凋亡 细胞迁移 肿瘤细胞 培养的 

分 类 号：R737.14[医药卫生—肿瘤]