水稻氮高效基因分子标记开发与基因型筛选  

Development of functional markers and genotype screening for nitrogen use efficiency genes in rice

作　　者：陶亚军 朱静妍 王军[1,3] 范方军[1,3] 许扬[1,3] 李文奇 王芳权 陈智慧[1,3] 蒋彦婕 朱建平[1,3] 李霞 杨杰 TAO Ya-Jun;ZHU Jing-Yan;WANG Jun;FAN Fang-Jun;XU Yang;LI Wen-Qi;WANG Fang-Quan;CHEN Zhi-Hui;JIANG Yan-Jie;ZHU Jian-Ping;LI Xia;YANG Jie(Jiangsu High Quality Rice Center/Nanjing Branch of Chinese National Center for Rice Improvement/Institute of Food Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,Jiangsu,China;College of Bioscience and Biotechnology,Yangzhou University,Yangzhou 225009,Jiangsu,China;Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Yangzhou University,Yangzhou 225009,Jiangsu,China)

机构地区：[1]江苏省农业科学院粮食作物研究所/国家水稻改良中心南京分中心/江苏省优质水稻工程技术研究中心,江苏南京210014 [2]扬州大学生物科学与技术学院,江苏扬州225009 [3]扬州大学/江苏省粮食作物现代产业技术协同创新中心,江苏扬州225009

出　　处：《作物学报》2022年第12期3045-3056,共12页Acta Agronomica Sinica

基　　金：江苏省重点研发计划项目(BE2020339);江苏省青年基金项目(BK20190255);国家青年科学基金项目(31901525);江苏省重点研发计划项目(BE2021374);国家自然科学基金国际(地区)合作与交流项目(31861143011);江苏省农业科技资助创新资金项目(CX(19)1002)资助。

摘　　要：氮素是促进水稻物质生产和产量形成的首要因素,其高效与合理的利用是农业可持续发展的重要保障。培育含有氮高效基因的水稻品种,充分发挥氮素高效吸收和利用遗传潜力是提高氮肥利用率、减少氮肥施用量的有效途径。本研究从氮素的吸收、转运、再分配和再利用等环节,选择了OsNR2、OsNPF6.1、OsTCP19、OsLHT1和OsGRF4共5个基因作为水稻氮高效遗传改良的基因组合,根据已报道的功能位点设计得到与目标基因共分离的基因功能标记,包括6对等位特异PCR标记和1对InDel标记,并对70份常规籼稻,34份常规粳稻和84份太湖资源水稻材料进行了鉴定。结果表明,OsNR2在籼稻中分布较广,OsNPF6.1、OsTCP19、OsGRF4在籼稻中分布较少,但是均未在常规粳稻中检出;常规粳稻中仅含有OsLHT1;同时我们还筛选出2份材料同时含有OsNR2、OsNPF6.1和OsGRF4高效单倍型。本研究开发的功能标记和筛选出的材料为通过分子标记辅助选择方法培育氮高效水稻新品种提供了技术支撑。Nitrogen is an essential mineral element that affects plants biomass and yield formation,and its efficient and reasonable utilization is an important guarantee for sustainable agricultural.Breeding rice varieties containing high nitrogen use efficiency(NUE)genes is an effective way to increase NUE and reduce the amount of nitrogen fertilizer.In this study,five genes,Os NR2,OsNPF6.1,Os TCP19,Os LHT1,and OsGRF4,were selected from the aspects of nitrogen absorption,transportation,and assimilation.Based on the reported functional haplotype,co-segregated markers were designed.Using six pairs of allele-specific PCR(AS-PCR)markers and one pair of InDel marker,70 indica rice,34 japonica rice,and 84 Taihu rice resources were identified.The results showed that Os NR2 was widely distributed in indica rice,while OsNPF6.1,Os TCP19,and OsGRF4 were less distributed.All 34 japonica rice and 84 Taihu rice resources only contained Os LHT1.We also successfully obtained two indica germplasms,which contained Os NR2,Os NPF6.1,and Os GRF4.The functional markers developed in this research and two materials provide technical support for breeding new rice varieties with high NUE through molecular marker-assisted selection(MAS)methods.

关 键 词：水稻 氮高效利用 等位特异PCR标记 INDEL标记 

分 类 号：S511[农业科学—作物学]