长链非编码RNA SRGAP3-AS2异常表达通过Janus激酶信号调节非小细胞肺癌侵袭和迁移  

SRGAP3-AS2 regulates non-small cell lung cancer cell proliferation, migration and invasion via JAK signaling pathway

作　　者：李新[1] 盖慧荣[1] 宋蕾[1] 张忠法 LI Xin;GAI Huirong;SONG Lei(Deparment of Radiation Therapy DivisionI,Qingdao Cancer Hospital,Qingdao 266042,China)

机构地区：[1]山东青岛市肿瘤医院放射治疗一科,266042

出　　处：《临床外科杂志》2022年第8期727-730,共4页Journal of Clinical Surgery

摘　　要：目的 探讨长链非编码RNA(LncRNAs)SRGAP3反义RNA 2(SRGAP3 antisense RNA 2,SRGAP3-AS2)通过JAK信号调控非小细胞肺癌(NSCLC)细胞的增殖、迁移和侵袭。方法 检测NSCLC组织及H23细胞SRGAP3-AS2表达,分析SRGAP3-AS2表达与NSCLC临床病理的关系。将H23细胞分为对照组、SRGAP3-AS2过表达组和SRGAP3-AS2过表达+JAK2激动剂组。检测各组细胞增殖、迁移、侵袭、MMP-2、Bcl-2及p-JAK2和p-STAT3的表达。结果 SRGAP3-AS2在NSCLC组织呈低表达,与淋巴结转移和TNM分期有关(P<0.05)。与对照组比,SRGAP3-AS2过表达组细胞活力、划痕愈合率、侵袭细胞数、MMP-2、Bcl-2、p-JAK2和p-STAT3表达减少(P<0.05);与SRGAP3-AS2过表达组比,SRGAP3-AS2过表达+JAK2激动剂组细胞活力、细胞划痕愈合率、侵袭细胞数、MMP-2、Bcl-2、p-JAK2和p-STAT3表达增加(P<0.05)。结论 NSCLC组织SRGAP3-AS2呈低表达,与淋巴结转移和TNM分期有关,过表达SRGAP3-AS2可抑制JAK2/STAT3信号,抑制NSCLC细胞H23增殖、迁移和侵袭。Objectives To elucidate the function and underlying mechanism of lncRNA(long non-coding RNA) SRGAP3-AS2(SRGAP3 antisense RNA 2) on the proliferation, migration and invasion of non-small cell lung cancer(NSCLC).Methods The expression of SRGAP3-AS2 in NSCLC tissues and H23 cells was detected, and the relationship between the expression of SRGAP3-AS2 and the clinicopathology of NSCLC was analyzed.H23 cells were divided into the NC group, pc-SRGAP3-AS2 group and pc-SRGAP3-AS2+JAK2 activator group.Cell proliferation, migration, invasion, MMP-2,Bcl-2,p-JAK2 and p-STAT3 expressions were detected in each group.Results The expression of SRGAP3-AS2 was lower in NSCLC tissues, which was related to lymph node metastasis and TNM stage(P<0.05).Compared with the NC group, the cell viability, the wound healing rate, the number of invasive cells, the expressions of MMP-2,Bcl-2,p-JAK2 and p-STAT3 in the pc-SRGAP3-AS2 group were decreased(P<0.05).Compared with the pc-SRGAP3-AS2 group, the cell viability, wound healing rate, number of invasive cells, MMP-2,Bcl-2,p-JAK2 and p-STAT3 expression in the pc-SRGAP3-AS2+JAK2 activator group increased(P<0.05).Conclusion The lower expression of SRGAP3-AS2 in NSCLC tissue is related to lymph node metastasis and TNM.Overexpression of SRGAP3-AS2 can inhibit JAK2/STAT3 signaling and inhibit the proliferation, migration and invasion of NSCLC cells H23.

关 键 词：SRGAP3-AS2 非小细胞肺癌 JAK信号 迁移 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]