人源性肾癌异体移植模型的建立及分析  

Establishment and analysis of patient-derived xenograft models of human renal cell carcinoma

作　　者：李锦楠 崔亮[1] 肖旭仁 吴学杰 代晓飞[1] 李景敏[1] LI Jinnan;CUI Liang;XIAO Xuren(Department of Urology,Ciuil Aviation General Hospital,Bejing 100123,China)

机构地区：[1]北京民航总医院泌尿外科,100123 [2]中国人民解放军总医院第三临床中心泌尿外科医学部 [3]江苏苏州明基医院泌尿外科

出　　处：《临床外科杂志》2022年第8期767-770,共4页Journal of Clinical Surgery

摘　　要：目的 构建人源性肾癌NOD-SCID小鼠皮下移植瘤模型及肾包膜下移植瘤模型(即原位移植瘤模型),对其生物学特征进行分析。方法 从术前诊断为肾癌的病人手术中获取活体组织标本,将其剪切后接种于NOD-SCID小鼠的背部皮下及原位肾包膜下,以制备原代移植瘤模型及传代移植瘤模型。观察移植瘤模型的成瘤时间和概率,肿瘤直径,是否出现侵袭和转移。结果 人源性肿瘤组织异种移植物(patient derived xenografts, PDX)移植瘤病人,男性5例,女性2例,其中肾透明细胞癌占6例,肾实质内多形性上皮样细胞肿瘤占1例。其中1例病人伴有肾静脉瘤栓。NOD/SCID小鼠移植瘤成瘤的平均潜伏期为63.83天,成瘤率为42.86%,移植瘤生长速度快慢不等,体积大小不一,为6.426~197.856 mm^(3)。对移植瘤分别做病理活组织检查,结果发现移植瘤与病人肾癌组织结构相近。结论 与移植方式相比,皮下移植瘤模型操作便捷,更容易评估、测量及观察肿瘤。原位移植瘤模型最大的优点在于其可以更好的模拟肾癌发生、发展的生物学行为。小鼠移植瘤与病人肾癌组织结构相似,PDX模型可作为肾癌临床前基础研究的有力工具。Objective Establishment of human renal cell carcinoma in NOD-SCID mice subcutaneous xenograft model and subcapsular of renal transplantation tumor model(for example, orthotopic transplantation tumor model),its biological characteristics were analyzed.Methods Tissue specimens were obtained from patients with clinically preoperative diagnosis of renal cell carcinoma.And then sheared the tissue to subcutaneous and orthotopic inoculated to NOD-SCID mice.To prepare xenograft models of primary and passage xenograft models.It was obseved of tumor xenograft model of the incubation period, the rate of tumor formation, tumor volume, tumor invasion and metastasis.Results PDX tumor patients, 5 males and 2 females, which accounted for 85.7%(6 cases) of renal clear cell carcinoma, renal parenchyma of pleomorphic epithelioid cell tumor accounted for 14.3%(1 case).One patient was with renal vein tumor thrombus.NOD/SCID mice transplanted tumor latent period was 63.83 days, the rate of tumor formation was about 42.86%,the growth rate of transplanted tumor was different, and the volume was different, for: 6.426-197.856 mm^(3).It was found that the transplanted tumor was similar to the human renal cell carcinoma from pathological changes of transplanted tumor.Conclusion Compared with orthotopic transplantation, subcutaneous transplantation tumor model was easy to operate, easy to evaluate, measure and observe the tumor.But orthotopic transplantation of the best advantages of the biological behavior of renal cell carcinoma which could better simulate the occurrence and development of the tumor.The transplanted tumor of mice was similar to the structure of human renal cell carcinoma, which could be used as a powerful tool for preclinical study of renal cell carcinoma.

关 键 词：肾细胞癌 动物模型 人源性肿瘤组织异种移植物 精准医学 个性化治疗 

分 类 号：R737.11[医药卫生—肿瘤]