蓝光对不同成熟期紫色彩椒采后果实花青素生物合成的影响  

Effects of blue light on anthocyanin biosynthesis in postharvest fruit of purple sweet pepper at different ripening stages

作　　者：王晓通 窦煜炜 陈晓璐[1] 李仪曼 栾恒 李岩[1,2,3] WANG Xiaotong;DOU Yuwei;CHEN Xiaolu;LI Yiman;LUAN Heng;LI Yan(College of Horticultural Science and Engineering,Shandong Agricultural University,Taian,Shandong 271018,China;Scientific Observing and Experimental Station of Environment Controlled Agricultural Engineering in Huang-Huai-Hai Region,Ministry of Agriculture and Rural Affairs,Shandong Agricultural University,Taian,Shandong 271018,China;State Key Laboratory of Crop Biology,Shandong Agricultural University,Taian,Shandong 271018,China)

机构地区：[1]山东农业大学园艺科学与工程学院,山东泰安271018 [2]山东农业大学农业农村部黄淮海设施农业工程科学观测实验站,山东泰安271018 [3]山东农业大学作物生物学国家重点实验室,山东泰安271018

出　　处：《植物生理学报》2022年第8期1507-1518,共12页Plant Physiology Journal

基　　金：山东省自然科学基金(ZR2020MC148);国家现代农业产业技术体系建设专项资金(CARS-23-C04);山东农业大学科技创新团队设施园艺优势团队(SYL2017YSTD07)。

摘　　要：为探究蓝光对彩椒(Capsicum annuum)花青素合成的作用机理,本研究以紫色彩椒‘紫水晶’为试材,采用便携式色差仪、超高效液相色谱-串联质谱联用法以及实时荧光定量PCR技术对不同成熟时期采后蓝光和白光照射不同时长的彩椒果皮色度值、花青素组分和含量以及花青素合成相关基因表达水平进行测定。结果表明,紫色彩椒采后果实富含的主要花青素种类为飞燕草素衍生物,包括飞燕草素-3-O-芸香糖苷、飞燕草素-3-O-鼠李糖苷和飞燕草素-3-O-葡萄糖苷。在绿熟期彩椒果皮中花青素含量最高,且该时期花青素合成下游结构基因表达水平最高;与白光相比,蓝光显著诱导花青素合成相关基因表达并促进花青素积累。通过对绿熟期采后蓝光处理的彩椒果皮花青素含量及其合成基因表达水平进行相关性分析,筛选出CaF3′5′H、CaANS、CaUFGT及CaMYB113为该品种彩椒花青素合成的关键基因。进一步对转录因子CaMYB113进行生物信息学分析,发现该转录因子的启动子区域包含G-box元件和MRE结合位点,推测其可能调控蓝光诱导的紫色彩椒花青素合成。综上,绿熟期紫色彩椒采后果实中CaMYB113能够通过响应蓝光信号,调控其他花青素合成关键基因表达,促进彩椒果皮中飞燕草素积累。To explore the mechanism of blue light on anthocyanin synthesis of sweet pepper(Capsicum annuum),the purple variety ‘Amethyst’ was used as plant material.The portable chroma meter,ultra-performance liquid chromatography–tandem mass spectrometry and real-time fluorescence quantitative PCR were used to determine the color value,anthocyanin composition and contents,as well as expression levels of genes related to anthocyanin synthesis in the skin of postharvest sweet pepper fruit,which were irradiated by blue and white light with various duration at different ripening stages.The results show that the main anthocyanins rich in postharvest fruit of purple sweet pepper are delphinidin derivatives,including delphinidin-3-O-rutinoside,delphinidin-3-O-rhethnoside and delphinidin-3-O-glucoside.The highest anthocyanin content was found in the skin of sweet pepper at green ripening stage,and the highest expression levels of structural genes downstream of anthocyanin synthesis were also detected during this period.Compared with white light,blue light could significantly increase the expression levels of genes related to anthocyanin synthesis and promote the accumulation of anthocyanin.Furthermore,CaF3′5′H,CaANS,CaUFGT and Ca MYB113 were screened as key genes involved in anthocyanin synthesis through correlation analysis of the anthocyanin content and their synthetic gene expression levels in skin of postharvest purple sweet pepper fruit treated with blue light during the green ripening stage.Further bioinformatics analysis of the transcription factor Ca MYB113 revealed that the promoter regions of this transcription factor contain G-box elements and MER binding sites,suggesting that it may regulate the synthesis of anthocyanin induced by blue light in purple sweet pepper.In summary,Ca MYB113 in postharvest fruit of purple sweet pepper at green ripening stage could promote the accumulation of delphinidin in fruit skin by responding to blue light signals and regulating the expression of other key genes,which involved

关 键 词：彩椒 蓝光 花青素 基因表达 CaMYB113 

分 类 号：S641.3[农业科学—蔬菜学]