核因子κB抑制物(IκB)家族在人肺腺癌中表达的生物信息学分析  

Bioinformatics analysis of IκB gene family expression in human lung adenocarcinoma

作　　者：唐铭 杨杰 袁青 徐文峰 谢翔 于红 TANG Ming;YANG Jie;YUAN Qing;XU Wenfeng;XIE Xiang;YU Hong(The School of Basic Medical Science,Southwest Medical University,Luzhou 646000,China)

机构地区：[1]西南医科大学基础医学院,四川泸州646000

出　　处：《细胞与分子免疫学杂志》2022年第8期727-735,共9页Chinese Journal of Cellular and Molecular Immunology

基　　金：泸州市科技计划项目资助(2017LZXNYD-J26)。

摘　　要：目的生物信息学方法筛选核因子κB抑制物(IκB)家族基因在肺腺癌(LUAD)中的差异表达基因(DEG),探究其在LUAD中的临床意义。方法采用R语言筛选出IκB家族基因的差异表达基因,并进行生存分析;R语言分析IκB家族基因表达与临床病理学特征的相关性;基于LinkedOmics对差异表达基因进行基因本体论(GO)富集、京都基因与基因组百科全书(KEGG)富集;肿瘤免疫评估资源(TIMER)分析差异表达基因与免疫细胞浸润的相关性;比例风险(COX)分析差异表达基因与LUAD预后的关系。结果IκB家族中核因子κB抑制物δ(NFKBID)基因和核因子κB抑制物ζ(NFKBIZ)基因在LUAD组织中表达显著降低,且NFKBID和NFKBIZ低表达的LUAD患者具有更短的生存期;NFKBID、NFKBIZ与肺腺癌T分期显著相关;富集分析显示NFKBID、NFKBIZ可能参与能量代谢、蛋白质表达和运输;NFKBID、NFKBIZ的表达与B细胞、CD4~+T细胞、中性粒细胞、树突状细胞(DC)的浸润呈正相关;COX分析提示NFKBIZ基因或可作为LUAD的独立预后因子。结论NFKBID、NFKBIZ在LUAD组织中低表达,且患者生存期短;NFKBID、NFKBIZ可能通过调节糖代谢和多种免疫细胞浸润参与LUAD的发生发展;NFKBIZ可能是LUAD的独立预后因子。Objective To explore the clinical significance of inhibitor of NF-κB(IκB)family in lung adenocarcinoma(LUAD)through bioinformatics analysis.Methods The differentially expressed genes of IκB family in LUAD were screened by R language for survival analysis.The correlation between the expression of IκB family genes and clinicopathological characteristics was analyzed by R language,and the genes related to survival rate were selected for further study.GO and KEGG enrichment analyses were performed with LinkedOmics.The infiltration of immune cells was analyzed with TIMER.The correlation between the candidate genes and the prognosis of LUAD was analyzed through COX model.Results The expression levels of NF-κB inhibitorδ(NFKBID)and NF-κB inhibitorζ(NFKBIZ)were significantly downregulated in tumor tissues,and the patients with low expression levels of NFKBID and NFKBIZ had shorter overall survival.NFKBID and NFKBIZ were significantly correlated with T stage of LUAD.Enrichment analysis showed that low expression levels of NFKBID and NFKBIZ were correlated with energy metabolism and protein expression and transport.The expression levels of NFKBID and NFKBIZ were positively correlated with the infiltration of B cells,CD4T cells,neutrophils,and dendritic cells.COX analysis indicated that NFKBIZ could be an independent prognostic factor for LUAD.Conclusion The expression levels of NFKBID and NFKBIZ were significantly downregulated in tumor tissues,and were correlated with overall survival.NFKBID and NFKBIZ could be involved in the occurrence and development of LUAD by regulating glycometabolism and multiple immune cells infiltration.NFKBIZ could be considered as an independent prognostic factor for LUAD.

关 键 词：肺腺癌 核因子κB抑制物(IκB) 生物信息学分析 

分 类 号：R734.2[医药卫生—肿瘤] Q811.4[医药卫生—临床医学] R392.12[生物学—生物工程]