长链非编码RNA ZNF667-AS1在非小细胞肺癌组织中表达上调  

Up-regulation of long non-coding RNA ZNF667-AS1in non-small cell lung cancer tissues

作　　者：梁海梅 欧宗兴[1] 王蕾 陈忠仁 LIANG Hai-mei;OU Zong-xing;WANG Lei;CHEN Zhong-ren(Pulmonary and Critical Care Medicine Department,Central South University Xiangya School of Medicine Affiliated Haikou Hospital,Hainan Haikou 570208,China)

机构地区：[1]中南大学湘雅医学院附属海口医院呼吸与危重症医学科,海南海口570208

出　　处：《内科急危重症杂志》2022年第4期305-307,311,共4页Journal of Critical Care In Internal Medicine

基　　金：海南省自然科学基金资助(No:818MS136)。

摘　　要：目的:探讨非小细胞肺癌(NSCLC)组织中长链非编码RNA(lncRNA)锌指蛋白667反义RNA1(ZNF667-AS1)的表达水平。方法:97例NSCLC患者被纳入本研究,术中留取新鲜的癌组织及对应癌旁组织,采用实时定量聚合酶链式反应(PCR)法检测lncRNA ZNF667-AS1和血管内皮生长因子(VEGF)的表达,分析lncRNA ZNF667-AS1的表达与NSCLC患者临床病理特征的关系,绘制受试者工作特征(ROC)曲线,评估lncRNA ZNF667-AS1用于诊断NSCLC的价值,分析癌组织中VEGF的表达与lncRNA ZNF667-AS1表达的相关性。结果:PCR检测结果显示,97例NSCLC患者癌组织中lncRNA ZNF667-AS1的平均相对表达量明显高于癌旁组织(P<0.05)。不同性别、年龄、肿瘤直径及病理组织类型的NSCLC患者癌组织中lncRNA ZNF667-AS1相对表达量比较,差异无统计学意义(P均>0.05),但不同组织分化程度、临床分期、浸润程度及有无淋巴结转移的患者癌组织中lncRNA ZNF667-AS1相对表达量比较,差异有统计学意义(P均<0.05)。实时荧光定量PCR检测结果显示,NSCLC癌组织中VEGF的相对表达量明显高于癌旁组织(P<0.01),NSCLC癌组织中lncRNA ZNF667-AS1的相对表达量与VEGF的表达呈正相关(P<0.05)。由ROC曲线可知,lncRNA ZNF667-AS1诊断NSCLC的AUC为0.792(95%CI:0.683-0.911,P<0.05),特异性为81.23%,敏感度为86.12%。结论:lncRNA ZNF667-AS1在NSCLC组织中表达上调,其可能作为一种促癌因子参与NSCLC的发生发展过程。Objective:To investigate the expression level of long non-coding RNA(lncRNA)zinc finger protein 667 antisense RNA1(ZNF667-AS1)in non-small cell lung cancer tissues.Methods:A total of 97 patients with NSCLC were included in this study.Fresh cancer tissues and corresponding paracancerous tissues were collected during surgery,and real-time quantitative polymerase chain reaction(PCR)was used to detect lncRNA ZNF667-AS1 and vascular endothelial growth factor(VEGF).The relationship between the expression of lncRNA ZNF667-AS1 and the clinicopathological characteristics of NSCLC patients was analyzed.The receiver operating characteristic(ROC)curve was drawn,and the value of lncRNA ZNF667-AS1 in the diagnosis of NSCL was evaluated.Correlation between lncRNA ZNF667-AS1 expression and VEGF expression in lung cancer tissues was analyzed.Results:The PCR results showed that the average relative expression of lncRNA ZNF667-AS1 in cancer tissues of 97 NSCLC patients was significantly higher than that in adjacent tissues(P<0.05).There was no significant difference in the relative expression of lncRNA ZNF667-AS1 in cancer tissues of NSCLC patients with different genders,ages,tumor diameters and pathological tissue types(all P>0.05).The relative expression of lncRNA ZNF667-AS1 in cancer tissues of patients with or without lymph node metastasis showed statistically significant difference(P<0.05).The results of real-time fluorescence quantitative PCR showed that the relative expression of VEGF in NSCLC cancer tissues was significantly higher than that in adjacent tissues(P<0.01),and the relative expression of lncRNA ZNF667-AS1 in NSCLC cancer tissues was positively correlated with the expression of VEGF(P<0.01).The area under the ROC curve(AUC)of lncRNA ZNF667-AS1 in the diagnosis of NSCLC was 0.792(95%CI:0.683-0.911,P<0.05),the specificity was 81.23%,and the sensitivity was 86.12%.Conclusion:The expression of lncRNA ZNF667-AS1 is up-regulated in NSCLC tissues,and it may be involved in the occurrence and development of NSCLC as a tum

关 键 词：非小细胞肺癌 长链非编码RNA ZNF667-AS1 

分 类 号：R734.2[医药卫生—肿瘤]