枸杞多糖对慢性青光眼大鼠视网膜神经节细胞损伤的改善作用及其可能机制  

Improvements of lycium barbarum polysaccharide on retinal ganglion cell damage in rats with chronic glaucoma and its possible mechanism

作　　者：蒋兆荣 段海霞 陈冰 王班伟 JIANG Zhao-rong;DUAN Hai-xia;CHEN Bing;WANG Ban-wei(Department of Ophthalmology,Zhuhai Second People′s Hospital,Zhuhai 519000,Guangdong,China)

机构地区：[1]珠海市第二人民医院(珠海市中西医结合医院)眼科,广东省珠海市519000

出　　处：《广西医学》2022年第16期1894-1900,共7页Guangxi Medical Journal

基　　金：广东珠海市医学科研基金项目(2016J017)。

摘　　要：目的探讨枸杞多糖(LBP)对慢性青光眼大鼠视网膜神经节细胞(RGC)损伤的改善作用,并基于Ras同源基因家族成员A(RHOA)/Rho相关卷曲螺旋形成蛋白激酶1(ROCK1)通路分析其作用机制。方法取100只大鼠建立慢性青光眼模型,将建模成功的80只大鼠随机分为模型组、LBP组、水仙环素组和LBP+水仙环素组,各20只。另取20只大鼠作为假手术组。建模成功后,分别给予LBP组、水仙环素组大鼠200 mg/kg LBP、1 mg/kg水仙环素灌胃,给予LBP+水仙环素组200 mg/kg LBP联合1 mg/kg水仙环素灌胃,给予模型组、假手术组大鼠蒸馏水灌胃,连续干预8周。于造模前、给药前、给药8周后测量各组大鼠的眼压。给药8周后取各组大鼠的右眼视网膜组织,观察视网膜组织形态,并检测视网膜组织中超氧化物歧化酶(SOD)活性、丙二醛和一氧化氮水平,RGC凋亡情况,以及视网膜中RHOA、ROCK1、Caspase-3、缺氧诱导因子1ɑ(HIF-1ɑ)、磷脂酰肌醇3-激酶(PI3K)、磷酸化PI3K(p-PI3K)、蛋白激酶B(AKT)、磷酸化AKT(p-AKT)的蛋白表达水平。结果(1)与假手术组相比,模型组大鼠视网膜组织变薄,细胞排列稀疏,膜盘间隙出现空泡,眼压升高、RGC凋亡数量增加,视网膜组织中丙二醇和一氧化氮水平、p-PI3K/PI3K比值和p-AKT/AKT比值均升高,视网膜组织中RHOA、ROCK1、Caspase-3、HIF-1ɑ蛋白表达水平上调,视网膜组织中SOD活性降低(均P<0.05)。(2)与模型组相比,LBP组大鼠视网膜组织增厚,细胞排列较整齐,膜盘间隙空泡减少,眼压降低、RGC凋亡数量减少,视网膜组织中丙二醇和一氧化氮水平、p-PI3K/PI3K比值和p-AKT/AKT比值均下降,视网膜组织中RHOA、ROCK1、Caspase-3、HIF-1ɑ蛋白表达水平下调,视网膜组织中SOD活性增高(均P<0.05);而水仙环素组大鼠视网膜组织结构紊乱、细胞排列稀疏,上述指标的变化与LBP组相反(均P<0.05)。(3)与LBP组相比,LBP+水仙环素组大鼠视网膜组织较薄,细胞排�Objective To investigate the improvements of lycium barbarum polysaccharide(LBP)on retinal ganglion cell(RGC)damage in rats with chronic glaucoma,and to analyze its mechanism based on Ras homolog family member A(RHOA)/Rho associated coiled-coil containing protein kinase 1(ROCK1)pathway.Methods One hundred rates were obtained to establish a chronic glaucoma model.A total of 80 rats with successful modeling were randomly assigned to model group,LBP group,narciclasine group or LBP+narciclasine group,with 20 rats in each group;additionally,20 rats were obtained as sham operation group.After successful modeling,intragastric administrations of 200 mg/kg LBP and 1mg/kg narciclasine were given to the LBP and narciclasine groups,respectively,of 200 mg/kg LBP combined with 1 mg/kg narciclasine to the LBP+narciclasine group,and of distilled water to the model and sham operation groups,for a 8-week continuous intervention.The intraocular tension in various groups was measured before modeling,before administration,8 weeks after administration.The retinal tissues of the right eye were obtained 8 weeks after administration,the morphology of the retinal tissues was observed,and the activity of superoxide dismutase(SOD),levels of malondialdehyde and nitric oxide,RGC apoptosis in the retinal tissues,and protein expressions of RHOA,ROCK1,Caspase-3,hypoxia-inducible factor 1α(HIF-1α),phosphatidylinositol-3-kinase(PI3K),phosphorylated PI3K(p-PI3K),protein kinase B(AKT),phosphorylated-AKT(p-AKT)in retinal tissues were detected.Results(1)Compared with the sham operation group,the rats in the model group presented thinner retinal tissues,sparse arrangement of the cells,appearance of vacuoles between the space of membrane and disc,an elevation of intraocular tension,increased number of RGC apoptosis,elevated levels of malondialdehyde and nitric oxide in retinal tissues,elevated ratios of p-PI3K/PI3K and p-AKT/AKT in retinal tissues,up-regulated protein expressions of RHOA,ROCK1,Caspase-3 and HIF-1αin retinal tissues,and a decreased ac

关 键 词：慢性青光眼 枸杞多糖 Ras同源基因家族成员A Rho相关卷曲螺旋形成蛋白激酶1 视网膜 神经节细胞 大鼠 

分 类 号：R775[医药卫生—眼科]