Cd(Ⅱ)对小球藻生长抑制和恢复研究  

Study on Cd(Ⅱ) on the Growth Inhibition and Recovery of Chlorella

作　　者：涂梦姿 尹鑫 赖发英 周春火[1,2] TU Mengzi;YIN Xin;LAI Faying;ZHOU Chunhuo(Jiangxi Province Agricultural Waste Resource Utlization and Non-point Source Pollution Prevention and Control Key Innovation Center of Integration of Industry and Education,Nanchang 330045,China;College of Land Resources and Environment,Jiangxi Agricultural University,Nanchang 330045,China)

机构地区：[1]江西省农业废弃物资源化利用与面源污染防控产教融合重点创新中心,江西南昌330045 [2]江西农业大学国土资源与环境学院,江西南昌330045

出　　处：《环境科学与技术》2022年第8期17-23,共7页Environmental Science & Technology

基　　金：国家自然基金项目:基于自养脱氮微生物的藻菌共生协同脱氮除磷机制(52160002);基于电极生物膜的厌氧氨氧化同步脱硝酸盐机制与工艺研究(21070757)。

摘　　要：重金属与小球藻相互作用的研究是认识和评估利用小球藻治理重金属污染有效性及生态风险的重要依据。该文通过序批式实验考察小球藻对重金属Cd的吸附行为,同时探究Cd胁迫对小球藻生长规律的影响。首先,实验结果表明小球藻对重金属的吸附是一个快速完成的过程,但随着Cd(Ⅱ)浓度的增大,其吸附平衡时间越长;其次,随着Cd(Ⅱ)浓度增大,对小球藻生长的抑制呈现逐渐增大的趋势,5 mg/L Cd(Ⅱ)胁迫下,小球藻对数期的5 d平均增长率相比对照减少了78.23%;再次,洗去胞外重金属后,小球藻处理组的生长速率均有恢复,5 mg/L Cd(Ⅱ)恢复期的5 d平均增长率相比对照增加了190%;最后通过透射电镜观察小球藻亚细胞结构发现经过5 d的恢复期有部分细胞器得到了恢复。The study of the interaction between heavy metals and Chlorella is an important basis for understanding and evaluating the effectiveness of using Chlorella to treat heavy metal pollution and ecological risks. This study investigates the adsorption behavior of Chlorella to heavy metal Cd through sequencing batch experiments, and at the same time explores the effect of Cd stress on the growth of Chlorella. The experimental results show that the adsorption of heavy metals by Chlorella is a rapid process, but as the concentration of Cd(Ⅱ) increases, the adsorption equilibrium time becomes longer. As the concentration of Cd(Ⅱ) increases, the inhibition of the growth of Chlorella showed a gradually increasing trend. Under the stress of 5 mg/L Cd(Ⅱ), the 5-day average growth rate of the logarithmic phase of Chlorella decreased by 78.23% compared with the control. The extracellular heavy metals were washed away, and the growth rate of the Chlorella treatment group was recovered. The growth rate during the recovery period of 5 mg/L Cd(Ⅱ) increased by 190% compared with the control.Finally, the subcellular structure of Chlorella was observed by transmission electron microscope(TEM), and it was found that after a 5-day recovery period, some organelles were recovered.

关 键 词：重金属胁迫 小球藻 生长规律 恢复 亚细胞结构 

分 类 号：X172[环境科学与工程—环境科学]