油菜与紫苏FAD3基因的序列比较分析  

Comparison Analyses of FAD3 Gene Sequences in Brassica napus and Perilla frutescens

作　　者：柏云会 张传利[1] 孙超[1] 杨加虎 张玉松 汪骞[2] 林良斌[1] Bai Yunhui;Zhang Chuanli;Sun Chao;Yang Jiahu;Zhang Yusong;Wang Qian;Lin Liangbin(College of Agronomy and Biotechnology,Yunnan Agriculture University,Kunming,650201;Institute of Horticultural Crops,Yunnan Academy of Agricultural Sciences,Kunming,650201)

机构地区：[1]云南农业大学农学与生物技术学院,昆明650201 [2]云南省农业科学院园艺作物研究所,昆明650201

出　　处：《分子植物育种》2022年第16期5236-5249,共14页Molecular Plant Breeding

基　　金：云南省油菜产业体系基金项目(2019KJTX005-05)资助。

摘　　要：以甘蓝型油菜‘滇早油16号’[亚麻酸(ɑ-Linolenic acid,ALA)含量为9.7%]和紫苏‘滇紫1号’(亚麻酸含量为64.7%)为材料,通过RT-PCR克隆开花后40 d种子的FAD3基因,分别获得了7条油菜和5条紫苏FAD3基因的CDS。油菜序列间存在着碱基缺失和碱基替换,其序列一致性为96.39%;紫苏序列间存在着碱基替换,无碱基缺失,其序列一致性为99.57%;但油菜和紫苏间的序列一致性仅为81.72%。油菜FAD3蛋白的等电点在7.42~7.85,偏中性,其氨基酸序列的一致性为97.00%;紫苏FAD3蛋白的等电点为8.93,偏碱性,其氨基酸序列的一致性为99.87%;但油菜和紫苏间的氨基酸序列一致性仅为83.33%。油菜和紫苏的FAD3蛋白均有3个跨膜区和3个组氨酸富集保守区。在组氨酸富集保守区有16个氨基酸改变,其中6个氨基酸发生性质改变。在第3组氨酸富集保守区和第3跨膜区,油菜与紫苏FAD3蛋白的二级结构存在较大的差异。这些结果暗示了油菜FAD3酶的比活力和紫苏的可能不同,是引起其种子ALA含量不同的原因之一,为进一步提高油菜亚麻酸含量提供理论基础。Using Brassica napus‘Dianzaoyou No.16’(ALA content 9.7%)and Perilla frutescens‘Dianzi No.1’(ALA content 64.7%)as materials,the FAD3 genes of 40-day-post-flowering seeds were cloned by RT-PCR,and the CDS of 7 rapeseed and 5 Perilla FAD3 genes were obtained.There are base deletions and base substitutions between rapeseed sequences,and their sequence identity is 96.39%;there are base substitutions between Perilla sequences without base deletions,and their sequence identity is 99.57%,but between rape and Perilla,the sequence identity is only 81.72%.The isoelectric point of rape FAD3 protein is neutral between 7.42 and 7.85,and its amino acid sequence identity is 97.00%;the isoelectric point of Perilla FAD3 protein is 8.93 slightly basic,and its amino acid sequence identity is 99.87%;but the amino acid sequence identity between rape and Perilla is only 83.33%.Both rape FAD3 protein and Perilla have three transmembrane regions and three histidine-rich conserved regions.There are 16 amino acid changes in the histidine-rich conserved region,of which 6 amino acids have changed properties.In the third histidine-enriched conserved region and the third transmembrane region,the secondary structure of rapeseed is quite different from that of Perilla.These results suggest that the specific activity of rapeseed FAD3 enzyme and Perilla may be different,which is one of the reasons for the different ALA content of its seeds,and provide a theoretical basis for further increasing the ALA content in Brassica napus.

关 键 词：甘蓝型油菜 紫苏 FAD3 Α-亚麻酸 

分 类 号：S565.4[农业科学—作物学] S567.219