基于代谢组学的不同桑品种桑叶菜品质比较研究  

Comparative Study on the Quality of Mulberry Leaf Vegetable from Different Mulberry Varieties Based on Metabolomics

作　　者：黄润生 詹润钧 陈壁煌 王溢 廖柏勇 张庆威 李超楠 Huang Runsheng;Zhan Runjun;Chen Bihuang;Wang Yi;Liao Boyong;Zhang Qingwei;Li Chaonan(College of Horticulture and Landscape Architecture,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;Guangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utllization,Guilin Guangxi 541006,China)

机构地区：[1]仲恺农业工程学院园艺园林学院,广州510225 [2]广西植物功能物质与资源持续利用重点实验室,广西桂林541006

出　　处：《蚕业科学》2022年第4期340-350,共11页ACTA SERICOLOGICA SINICA

基　　金：广东省教育厅青年科技创新项目(2019KQNCX50);广东省自然科学基金面上项目(2019A1515011137);广西植物功能物质与资源持续利用重点实验室开放基金项目(FPRU2022-6);广东省现代农业产业技术体系创新团队项目(2020KJ137)。

摘　　要：以粤椹大10和粤桑11号2个桑品种桑枝顶端较嫩的一芽两叶为原材料制作加工桑叶菜,测定2种桑叶菜的维生素、总多糖和总黄酮含量,并采用超高效液相色谱-串联质谱(UPLC-MS/MS)技术对2种桑叶菜进行代谢组学比较研究。结果表明:2种桑叶菜中的维生素、总多糖和总黄酮含量差异明显,粤桑11号桑叶菜与粤椹大10桑叶菜相比,共筛选出147种差异代谢物,主要包括黄酮类、酚酸类、萜类、生物碱类、醌类及其他类等,其中黄酮类化合物是2种桑叶菜间数量相差最大的一类物质。综合营养成分的差异与代谢组学的结果,认为2种桑叶菜可能存在不同的代谢调控模式,粤桑11号的桑叶菜品质更佳,可能更适合作为菜用桑树品种。Based on ultra high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS), comparative metabolomic studies were conducted on two mulberry leaf vegetables processed with one bud and two leaves grown on the branch top of mulberry variety Yueshenda 10 and Yuesang 11. The contents of vitamins, total polysaccharides and total flavonoids in the two mulberry leaf vegetables were determined. The results showed that the contents of vitamins, total polysaccharides and total flavonoids in the two mulberry leaf vegetables were significantly different. A total of 147 differential metabolites were screened in mulberry leaf vegetable of Yuesang 11 when compared with that of Yueshenda 10, including flavonoids, phenolic acids, terpenoids, alkaloids, quinones and others, among which flavonoids were the most different one between the two mulberry leaf vegetables. The difference in nutritional components and the results of metabolomics indicated that the two mulberry leaf vegetables might have different metabolic regulation modes. The mulberry leaf vegetable of Yuesang 11 has high quality, suggesting that Yuesang 11 might be more suitable to be used as a vegetable mulberry variety.

关 键 词：桑叶菜 代谢组 营养成分 差异代谢物 

分 类 号：S886.9[农业科学—特种经济动物饲养]