脑胶质瘤靶向SPIO-PLA-P53分子探针的构建及磁共振成像实验研究  

Preparation of targeting SPIO-PLA-P53 glioma molecular probe and its application in magnetic resonance imaging

作　　者：段丽珍 郭莉莉[1] 柏根基[1] DUAN Lizhen;GUO Lili;BO Genji(Department of Medical Imaging,the Affiliated Huai’an No.1 People's Hospital of Nanjing Medical University,Huai'an 223300,China)

机构地区：[1]南京医科大学附属淮安第一医院影像中心,江苏淮安223300

出　　处：《南京医科大学学报（自然科学版）》2022年第9期1223-1229,共7页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京医科大学科技发展基金基金(2016NJMU ZD085)。

摘　　要：目的:探讨靶向磁共振SPIO-PLA-P53分子探针对大鼠脑胶质瘤的诊断效能。方法:合成超顺磁性氧化铁纳米粒子SPIO-PLA和脑胶质瘤靶向纳米分子探针SPIO-PLA-P53。使用透射电镜和动态光散射检测分子探针的物理性质;CCK-8(Cell Counting Kit-8)法检测细胞活性;并检测分子探针体外透过血脑屏障的效能。利用7.0 T小动物专用磁共振仪及配套线圈检测纳米探针在胶质瘤中的分布。对荷瘤鼠脑组织进行病理染色,明确靶向分子探针在肿瘤组织中的蓄积。使用GraphPad Prism软件对结果进行统计学分析。结果:脑胶质瘤靶向SPIO-PLA-P53纳米粒子大小均匀,分散度较好,具有良好的生物相容性及靶向聚集效应。磁共振扫描结果显示荷瘤大鼠在注射靶向SPIO-PLA-P53探针后瘤体T2WI信号减低,在注射SPIO-PLA探针溶液后瘤体信号无明显变化。病理结果证实注射SPIO-PLA-P53大鼠胶质瘤内大量铁离子蓄积。结论:SPIO-PLA-53可作为大鼠脑胶质瘤特异性显像的磁共振分子探针。Objective:This study aims to investigate the diagnostic efficacy of targeted magnetic resonance SPIO-PLA-P53 molecular probe in rat glioma.Methods:Superparamagnetic iron oxide nanoparticles SPIO-PLA and glioma targeting nanomolecular probe SPIO-PLA-P53 were synthesized.The physical properties of molecular probes were detected by transmission electron microscopy and dynamic light scattering.Cell activity was detected by Cell Counting Kit-8(CCK-8)method.The penetration efficiency of molecular probe through blood-brain barrier in vitro was detected.The distribution of nanoprobes in glioma was detected by 7.0 T small animal-special magnetic resonance instrument and matching coil.Pathological staining of tumor-bearing mouse brain tissue was performed to clarify the accumulation of targeted molecular probes in the tumor tissue.Statistical analysis of the results was performed using GraphPad Prism software.Results:Glioma targeted SPIO-PLA-P53 nanoparticles have uniform size,good dispersion,good biocompatibility and targeted aggregation effect.Magnetic resonance scanning showed that the tumor T2WI signal was decreased after injection of targeted SPIO-PLA-P53 probe in the tumor-bearing rats,and there was no significant change in the tumor signal after injection of SPIO-PLA probe solution.The pathological results confirmed that a large amount of iron ions accumulated in the glioma of rat injected with SPIO-PLA-P53.Conclusion:SPIO-PLA-P53 can be used as an magnetic resonance molecular probe for specific imaging of rat gliomas.

关 键 词：脑胶质瘤 SPIO 磁共振成像 分子探针 P53 

分 类 号：R739.41[医药卫生—肿瘤]