酸性乳糖酶活测定及对奶粉中乳糖水解条件的研究  

Determination of Acid Lactase Activity and Study on Hydrolysis of Lactose in Milk Powder

作　　者：何爱红 李雪飞 王义哲 赵喜红 HE Aihong;LI Xuefei;WANG Yizhe;ZHAO Xihong(Beijing Shijishengming Medical Science and Technology Co.,Ltd.,Beijing 100000,China;School of Environmental Ecology and Biological Engineering,Wuhan Institute of Technology,Wuhan 430205,China)

机构地区：[1]北京世纪盛名医学科技有限公司,北京100000 [2]武汉工程大学环境生态与生物工程学院,湖北武汉430205

出　　处：《食品安全导刊》2022年第21期57-60,64,共5页China Food Safety Magazine

摘　　要：目的:测定奶粉中酸性乳糖酶的活性并优化其分解条件。方法:采用《食品药品法典》(第五版)方法测定乳糖酶的酶活力,采用高效液相色谱法测定水解前后奶液中的乳糖含量,并优化酶解反应条件。结果:不同批次产品乳糖酶酶活力基本稳定,酶活力值最大值为8050 ALU·g^(-1)。当酶解温度为55℃、酶解时间为120 min、酶解pH值为4时,乳糖酶的分解效率最高,达到91%。结论:本研究可为含乳糖酶的乳制品和直接服用的乳糖酶产品开发提供一定的理论基础。Objective:To determine acid lactase activity and optimize its decomposition conditions in milk powder.Method:The activity of lactase was determined by“Food and Drug Code Fifth Edition”,and the content of lactose in milk before and after hydrolysis was determined by HPLC,and the enzymatic hydrolysis reaction conditions were optimized.Result:The results showed that the enzyme activities of lactase were basically stable in different production batches,and the maximum enzyme activity value was 8050 ALU·g^(-1).In addition,this study determined that under the acidic environment with reaction temperature of 55℃,reaction time of 120 min and pH value of enzymatic hydrolysis reaction environment of 4,the enzymatic hydrolysis efficiency of lactase was the highest,which can improve the decomposition efficiency to 91%,and has good enzymatic hydrolysis activity.Conclusion:This study was expected to provide a theoretical basis for the development of lactase containing dairy products and directly taken lactase products.

关 键 词：乳糖酶 酶解效率 单因素试验 高效液相色谱 

分 类 号：TS252.51[轻工技术与工程—农产品加工及贮藏工程]