Flexible electrostatic hydrogels from marine organism for nitric oxide-enhanced photodynamic therapy against multidrug-resistant bacterial infection  

海洋生物源静电水凝胶实现一氧化氮增强光动力治疗以对抗多药耐药菌感染

作　　者：Yujie Sun Rong-Lai Wen Dan Yu Yiwen Zhu Liang Zheng Xiangdong Liu Haoran Wang Bingran Yu Fu-Jian Xu 孙玉洁;闻荣来;余丹;朱艺文;郑良;刘向东;王浩然;俞丙然;徐福建(State Key Laboratory of Chemical Resource Engineering,Key Lab of Biomedical Materials of Natural Macromolecules(Beijing University of Chemical Technology,Ministry of Education)and Laboratory of Biomedical Materials,College of Materials Science and Engineering,Beijing University of Chemical Technology,Beijing 100029,China;School of Materials Science and Engineering,Zhejiang Sci-Tech University,Xiasha Higher Education Zone,Hangzhou 310018,China)

机构地区：[1]State Key Laboratory of Chemical Resource Engineering,Key Lab of Biomedical Materials of Natural Macromolecules(Beijing University of Chemical Technology,Ministry of Education)and Laboratory of Biomedical Materials,College of Materials Science and Engineering,Beijing University of Chemical Technology,Beijing 100029,China [2]School of Materials Science and Engineering,Zhejiang Sci-Tech University,Xiasha Higher Education Zone,Hangzhou 310018,China

出　　处：《Science China Materials》2022年第10期2850-2860,共11页中国科学（材料科学（英文版）

基　　金：supported by the National Key R&D Program of China(2021YFB3800900);the National Natural Science Foundation of China(22122501,21875014 and 52073013);Beijing Outstanding Young Scientist Program(BJJWZYJH01201910010024)。

摘　　要：The abuse of antibiotics in treating microbial infections has led to the emergence and prevalence of drugresistant bacteria.Thus,the development of novel antibacterial materials is attracting increasing attention.Here,a series of flexible electrostatic hydrogels with excellent antibacterial ability were constructed using a mixture of nitric oxide(NO)-releasing nitrated chitosan(CSNO)and mesotetra(4-carboxyphenyl)porphine(TCPP)with salmon sperm DNA(ssDNA)solution.When cultured with gram-negative bacteria under solar simulator irradiation,TCPP-CSNO_(m)ssDNA_(n) hydrogels released reactive oxygen species(ROS)and NO to produce peroxynitrite ions(ONOO^(−)).ONOO−is efficient at killing bacteria,thereby improving the antimicrobial ability of photodynamic therapy against gram-negative bacteria.The hydrogels exhibited powerful antibacterial activity in vivo when used to treat skin infections caused by drugresistant bacteria,making them a promising candidate for clinical applications.A string of antibacterial hydrogels that release ROS and NO synergistically can bring new possibilities for effectively killing drug-resistant bacteria and be of great value in anti-infection wound dressings and other applications.在治疗微生物感染疾病方面滥用抗生素提高了耐药菌的致病率.本研究提出了一种利用硝化壳聚糖与鱼精DNA包裹光敏剂构建静电水凝胶(TCPP-CSNO_(m)-ssDNAn)的策略.在模拟太阳光的照射下,水凝胶TCPP-CSNO_(m)-ssDNA_(n)释放一氧化氮与活性氧物质,进而形成具有强氧化活性的过氧亚硝酸盐离子(OONO^(−)),实现了高效协同杀灭多药耐药革兰氏阴性菌的作用,改善了单一的光动力治疗对革兰氏阴性菌效果不佳的现状.在用于治疗由多药耐药的铜绿假单胞菌引起的皮肤感染时,水凝胶TCPP-CSNO_(2)-ssDNA1在体内表现出有效的抗菌作用,同时具有生物安全性,这使其在抗感染伤口敷料领域具有巨大的应用潜力.

关 键 词：electrostatic hydrogel drug-resistant bacteria PEROXYNITRITE photodynamic therapy 

分 类 号：TQ427.26[化学工程] R318.08[医药卫生—生物医学工程]