SS31肽改善快速老化小鼠SAMP8认知功能及相关机制的研究  

SS31 peptide improves the cognitive function of SAMP8 in rapidly aging mice and its related mechanism

作　　者：霍甜甜 白文楼 李娜[1] 罗欣彤 赵景茹[1] 贾砚秋[1] 孟楠[1] 王建华[1] Huo Tiantian;Bai Wenlou;Li Na;Luo Xintong;Zhao Jingru;Jia Yanqiu;Meng Nan;Wang Jianhua(Department ofNeurology,the Hebei People's Hospital,Shijiazhuang050051,China)

机构地区：[1]河北省人民医院神经内科,石家庄050051

出　　处：《脑与神经疾病杂志》2022年第9期539-543,共5页Journal of Brain and Nervous Diseases

基　　金：河北省教育厅创新资助项目(CX2ZBS2019115)。

摘　　要：目的 探讨SS31肽对快速老化小鼠SAMP8认知的改善作用以及对海马区神经元的线粒体形态及氧化应激产物的影响。方法 将8个月龄SAMP8小鼠随机分为3组:5mg SS31腹腔注射组(SS31组),生理盐水腹腔注射组(SAMP8-V组),模型组(SAMP8组)。以8个月龄SAMR1小鼠作为空白对照组。连续腹腔注射给药8w后,采用透射电镜方法检测各组小鼠海马区线粒体的形态,免疫组化检测各组小鼠海马区内4-羟基壬烯醛(4-hydroxynonenal,4-HNE)的含量,Western blot法检测各组小鼠海马区内线粒体分裂相关蛋白1 (fission1,Fis1)的表达。结果 与SAMP8组和SAMP8-V组相比,SS31干预组小鼠认知功能得到明显改善,海马区内线粒体形态无明显肿胀,嵴量较多,内部结构完整,Fis1蛋白表达明显下降,4-HNE表达明显降低(P<0.05)。结论 SS31肽可能主要通过减轻海马区神经元内的氧化应激反应及减少线粒体分裂达到改善AD模型小鼠SAMP8的认知功能的作用。Objective To investigate the effect of SS31 peptide on improving the cognition of SAMP8 in rapidly aging mice and its effect on the morphology of mitochondria and the oxidative stress product of hippocampal neurons.Method 8-month-old SAMP8 mice were randomly assigned into three groups:5 mg SS31 injection group(SS31 group),saline injection group(SAMP8-V group),model group 10(SAMP8 group),and 8-month-old SAMR1 mice were used as the sham group(SAMR1).After 8 weeks of continuous intraperitoneal injection,the morphology of mitochondria in the hippocampus of different groups of mice were detected by electron microscopy,and the content of 4-hydroxynonenal in the hippocampus of different groups of mice were detected by immunohistochemistry.Fis1 expression was observed by Western blot.in the hippocampus of different groups of mice.Results Compared with the SAMP8 group and the SAMP8-V group,the cognitive function of the mice in the SS31 intervention group was significantly improved;there was no obvious swelling of mitochondria in the hippocampus,the number of cristae was large and the internal structure was complete of the mitochondria;the expression of Fisl and the 4-HNE were significantly decreased(P<0.05).Conclusions SS31 peptide could improve the cognitive function SAMP8 mice by reduce the oxidative stress and mitochondrial division in the neurons of hippocampus.

关 键 词：阿尔茨海默病 氧化应激 SS31肽 线粒体分裂 

分 类 号：R749.1[医药卫生—神经病学与精神病学]