基于低场核磁共振技术快速测定油茶籽含油率  

Rapid detection of oil contentin in Camellia oleifera seeds based on low field nuclear magnetic resonance

作　　者：孔凡利 付宇新[2,3] 王召滢 曹冰[2,3] 贺义昌 曾凡新[2] 雷小林 Kong Fanli;Fu Yuxin;Wang Zhaoying;Cao Bing;He Yichang;Zeng Fanxin;Lei Xiaolin(Academy of Forestry Inventory and Planning,National Forestry and Grassland Administration of P.R.C.,Beijing 100714,China;Jiangxi Academy of Forestry,Nanchang Jiangxi 330013,China;Jiangxi Key Laboratory of Forest Cultivation,College of Forestry,Jiangxi Agricultural University,Nanchang Jiangxi 330045,China)

机构地区：[1]国家林业和草原局林草调查规划院,北京100714 [2]江西省林业科学院,江西南昌330013 [3]江西农业大学林学院·江西省森林培育重点实验室,江西南昌330045

出　　处：《南方林业科学》2022年第4期70-74,共5页South China Forestry Science

基　　金：江西省林业科学院重大科技项目(项目编号:2018512101)。

摘　　要：为了快速准确地测定油茶籽含油率,解决传统测定方法费时费力等问题,提出一种基于低场核磁共振技术的油茶籽含油率快速测定方法。以油茶籽作为试验对象,采用NMI20低场核磁共振仪测定油茶籽含油率,与索氏提取法结果进行比较分析,并考察方法的精密度和稳定性。结果表明:核磁共振法不同时间定标油茶籽油和水分标准曲线的拟合相关系数均大于0.999,核磁共振信号响应值相近,油茶籽油和水分定标曲线表现稳定,可重复性好。两种方法测定的30份样品含油率结果绝对偏差均小于2%,平均相对偏差均小于3%。核磁共振法试验精密度和稳定性结果,20份样品7次重复测定含油率结果标准偏差范围为0.10%~0.24%,均小于0.3%;变异系数范围为0.34%~0.91%,均在1.0%以内。20份样品7次测定含油率结果标准偏差范围为0.58%~2.43%,均小于3%;变异系数范围为1.47%~9.14%,均在10%以内,稳定性好。因此,核磁共振法可作为快速、无损、准确测定油茶籽含油率的测定方法。In order to quickly and accurately detect the oil content of Camellia oleifera seed and solve the time-consuming and laborious problems of traditional detection methods.A method for quick detecting the oil content based on low field nuclear magnetic resonance(LF-NMR).C.oleifera seeds are used as test objects,and the oil content is determined by the soviet extract(SE)and LF-NMR method,compare the results,and examining the precision and stability of the method.The results shows that the fitting correlation coefficients of the calibration curves of C.oleifera seed oil and moisture at different times were all greater than 0.999,and the NMR signal response values were similar.The calibration curves of C.oleifera seed oil and moisture showed stable performance and good repeatability.The absolute deviation and average relative deviation of the two methods were all less than 2%and 3%,respectively.Precision and stability of NMR tests,the SD of oil content of 20 C.oleifera seeds was 0.10%~0.24%,all less than 0.3%,and the CV was 0.34%~0.91%,all within 1.0%.The SD range of oil content of 20 samples for 7 times was 0.58%-2.43%,all less than 3%,and the CV range was 1.47%~9.14%,all within 10%,indicating good stability.Therefore,NMR method can be used as a detection method for fast,non-destructive,and accurate metering of C.oleifera seeds.

关 键 词：核磁共振 索氏提取 油茶籽 含油率 

分 类 号：S794.4[农业科学—林木遗传育种] TQ646[农业科学—林学]