基于光子晶体的硝化甘油荧光传感器的制备  

Preparation of Nitroglycerine Sensor Based on Photonic Crystal

作　　者：刘占芳 张冠男 孙振文 胡灿 周红 乔婷 李露 朱军 LIU Zhanfang;ZHANG Guannan;SUN Zhenwen;HU Can;ZHOU Hong;QIAO Ting;LI Lu;ZHU Jun(Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China;Shaanxi University of Science and Technology,Xi’an 710021,China)

机构地区：[1]公安部鉴定中心,北京100038 [2]陕西科技大学,西安710021

出　　处：《刑事技术》2022年第5期471-476,共6页Forensic Science and Technology

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(2021JB011);刘耀院士专项资助项目(2016-YZ-01)。

摘　　要：本文通过光子晶体荧光传感器的制备,实现有机炸药硝化甘油的快速检测和半定量分析。首先以聚苯乙烯@二氧化硅核壳结构纳米微球为基体,自组装制备二氧化硅空心球光子晶体,并在合适波长位置光子带隙中掺入罗丹明6 G荧光探针溶液,形成光子晶体荧光传感器。在波长为365 nm的激发光照射下,荧光探针因为受到光子带隙的带边和慢光子效应,发出放大的荧光信号,而硝化甘油溶液对于荧光信号具有淬灭作用,通过淬灭响应的分析,进行硝化甘油样品的快速检测。结果表明,本文研究制备的一套能够增强荧光探针信号的光子晶体功能体系,通过荧光强度变化实现对有机炸药硝化甘油的高灵敏检测。当溶液中存在硝化甘油爆炸物时,荧光探针的信号被有效淬灭,根据不同浓度样品其淬灭程度不同的原理,实现硝化甘油的半定量检测。荧光检测技术具有灵敏度高、选择性好、响应速度快、稳定性高以及操作简单等优点,光子晶体荧光传感器普适性较好,可以通过筛选特异性的荧光探针或者传感阵列交互响应等策略提高灵敏度,在法庭科学领域现场爆炸物痕量快速检测方面具有良好的应用前景。Nitroglycerine(NG),also known as glyceryl trinitrate,can be used to make explosives and gunpowder.The NG-derived explosive is usually of great energetic power.At present,nitroglycerin is mainly determined with gas/liquid chromatography-mass spectrometry.However,nitroglycerin is prone to complete explosion,high heat sensitivity and easy volatilization,resulting in the existing laboratory technology difficult to detect the NG-derived explosive eligibly.Fortunately,fluorescence detection can have the NG-drived explosive residues determined sensitively and rapidly.Here,a set of photonic crystal functional system was prepared to get the designed probe enhancing its emitting fluorescent signal that was yet able to quench with NG or substance from organic NG-explosive,hence realizing the highly sensitive detection of NG or organic NG-explosive through discrimination of the changing fluorescence intensity.With the core-shell nanosphere of polystyrene@silica as matrix,the silica hollow-sphere photonic crystals were prepared under self-assembly,then doped of Rhodamine 6G fluorescence probe solution into the photonic band gap at the appropriate wavelength to form a photonic crystal fluorescence sensor,finally.Excitated through the 365 nm-wavelength light,the fluorescent probe will emit an amplified fluorescent signal owing to the band edge and slow photon effect of the photonic band gap,whereas a quenching effect would be appearing on the fluorescent signal if nitroglycerin was present in the solution of probe.Through analysis of the quenching response,nitroglycerin or its sample can be detected quickly,and so can NG-derived explosive.According to different quenching effect of concentration-various samples,nitroglycerin and/or NG-derived explosive can be realized of semi-quantitative detection.Fluorescence detection has the advantages of high sensitivity,good selectivity,fast response speed,high stability and simple operation.Photonic crystal fluorescence sensor is of good universality,capable of improving its sensitivity v

关 键 词：光子晶体 硝化甘油 荧光传感器 空心球 

分 类 号：DF794.3[政治法律—诉讼法学]