microRNA-93靶向作用WASF3对乳腺癌转移抑制的机制  

Inhibitory mechanism of microRNA-93 targeting WASF3 on breast cancer metastasis

作　　者：王茂[1] 宫小勇[2] 邱春丽 吴少锋[1] 郑伟[2] 雷毅[2] 代引海[1] WANG Mao;GONG Xiao-yong;QIU Chun-li;WU Shao-feng;ZHENG Wei;LEI Yi;DAI Yin-hai(Cancer Breast Surgery,Second Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine,Xianyang 712000,China;Department of Urology,Second Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine,Xianyang 712000,China;Department of Oncology,Xianyang Central Hospital,Shaanxi Province,Xianyang 712000,China)

机构地区：[1]陕西中医药大学第二附属医院肿瘤乳腺外科,陕西咸阳712000 [2]陕西中医药大学第二附属医院泌尿科,陕西咸阳712000 [3]陕西省咸阳市中心医院肿瘤科,陕西咸阳712000

出　　处：《中国现代普通外科进展》2022年第9期679-683,共5页Chinese Journal of Current Advances in General Surgery

基　　金：陕西省卫生计生委项目(2016D031);咸阳市2016年科学技术研究计划项目(2016k02-94);陕西省中医药管理局共建项目(2021-GJ-LC012)。

摘　　要：目的:探讨微小RNA(miR-93)靶向作用WASF3对乳腺癌转移抑制作用及可能的作用机制。方法:培养SHZ-88乳腺癌细胞株,划痕愈合实验、Transwell小室检测miR-93对SHZ-88细胞转移、侵袭能力的影响;荧光报告基因实验及Western blot技术检测miR-93对WASF3表达的影响。结果:乳腺癌组织中miR-93的表达低于癌旁组织,WASF3的表达高于癌旁组织,差异均有统计学意义(P<0.05)。与对照组相比,miR-93上调组、WASF3下调组SHZ-88细胞穿过小室微孔膜的细胞数、细胞划痕间愈合距离均显著减小,miR-93下调组、WASF3上调组SHZ-88细胞穿过小室微孔膜的细胞数、细胞划痕间愈合距离均显著增大,差异均有统计学意义(P<0.05)。双荧光素酶活性测定结果显示,野生型组miR-93-3′-UTR的荧光活性低于阳性对照组(P<0.05)。与阳性对照组比较,模拟物组WASF3表达明显降低,抑制物组WASF3表达明显升高,差异均有统计学意义(P<0.05)。结论:上调miR-93可抑制SHZ-88细胞增殖、转移能力;该作用机制可能与直接靶向调节介导WASF3的表达有关。Objective:To investigate inhibitory effect of microRNA(miR-93)targeting WASF3 on breast cancer metastasis and its possible mechanism.Methods:SHZ-88 breast cancer cell line was cultured,and the effects of miR-93 on the metastasis and invasion ability of SHZ-88 cells were detected by scratch test and Transwell chamber assay.Results:The expression of miR-93 in breast cancer tissue was lower than that in adjacent tissue,and the expression of WASF3 was higher than that in adjacent tissue,the differences were statistically significant(P<0.05).Compared with the control group,the number of cells passing through the microporous membrane and the healing distance between cell scratches in SHZ-88 cells in the miR-93 up-regulation group and WASF3 down-regulation group were significantly reduced.The number of cells passing through the microporous membrane of the chamber and the healing distance between cell scratches of-88 cells were significantly increased,and the differences were statistically significant(P<0.05).The fluorescence activity of 93-3′-UTR was lower than that of the positive control group(P<0.05).Compared with the positive control group,the expression of WASF3 in the mimic group was significantly decreased,and the expression of WASF3 in the inhibitor group was significantly increased,with statistical significance(P<0.05).Conclusion:Up-regulation of miR-93 can inhibit the proliferation and metastasis of SHZ-88 cells,and the mechanism may be related to the direct targeting and regulation of WASF3 expression.

关 键 词：MicroRNA-93 靶基因 WASF3 乳腺肿瘤 

分 类 号：R737.9[医药卫生—肿瘤]