6种代表性沉香精油的化学成分及抗氧化、抗炎活性比较分析  

Comparative analysis of chemical constituents and anti-oxidant and anti-inflammatory activities of six representative agarwood essential oils

作　　者：陈细钦 王灿红 冯剑[2] 陈德力[2] 魏建和 刘洋洋[2] CHEN Xi-qin;WANG Can-hong;FENG Jian;CHEN De-li;WEI Jian-he;LIU Yang-yang(National Engineering Laboratory for Breeding of Endangered Medicinal Materials,Ministry of Education of Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China;Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization,Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine,Hainan Branch of Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Haikou 570311,China)

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,中草药物质基础与资源利用教育部重点实验室,濒危药材繁育国家工程实验室,北京100193 [2]中国医学科学院北京协和医学院药用植物研究所海南分所,海南省南药资源保护与开发重点实验室,国家中医药管理局沉香可持续利用重点研究室,海南海口570311

出　　处：《中草药》2022年第18期5720-5730,共11页Chinese Traditional and Herbal Drugs

基　　金：国家重点研发计划项目(2018YFC1706403);海南省重点研发计划项目(ZDYF2020163,ZDYF2020111);中国医学科学院医学与健康科技创新工程项目(2021-I2M-1-032);国家现代农业产业技术体系项目(CARS-21)。

摘　　要：目的 比较不同来源、不同提取方式的6种沉香精油化合物组成、抗氧化能力及抗炎活性的差异。方法 采用气相色谱-飞行时间质谱联用仪(gas chromatography-time of flight mass spectrometry,GC-TOF MS)对6种沉香精油的化学成分进行分析,并测定其清除1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-trinitrophenylhydrazine,DPPH)与2,2-氮杂双-(3-乙基苯并噻唑啉-6-磺酸)二铵盐[2,2-azabis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt,ABTS]自由基的能力,考察其对脂多糖(lipopolysaccharide,LPS)诱导的小鼠单核巨噬细胞RAW264.7活力的影响。结果 从通体香水蒸气蒸馏精油(TTS)、通体香超临界萃取精油(TTC)、板头香水蒸气蒸馏精油(BTS)、板头香超临界萃取精油(BTC)、奇楠沉香水蒸气蒸馏精油(QNS)和奇楠沉香超临界萃取精油(QNC)中鉴定出的化合物数量分别为48、55、59、71、72和35种,所含香味物质小分子芳香物质及倍半萜类成分相对含量之和分别为81.42%、87.84%、89.03%、62.05%、63.83%和28.36%,所含2-(2-苯乙基)色酮类化合物相对含量分别为0、0、0.25%、22.42%、17.48%、60.42%。6种沉香精油均具有清除DPPH和ABTS自由基能力,且质量浓度在0.5~5 mg/m L与自由基清除能力均呈正相关。3种水蒸气蒸馏沉香精油(BTS、TTS、QNS)对LPS诱导RAW264.7细胞的半数抑制浓度(half inhibitory concentration,IC50)分别为18.61、30.30、18.05μg/m L,而3种超临界CO_(2)萃取沉香精油(BTC、TTC、QNC)的IC50值分别为41.98、38.45、24.50μg/m L。结论 超临界萃取沉香精油同水蒸气蒸馏沉香精油化合物组成相差较大,其能够提取出更多的脂肪酸类和色酮类化合物;板头香和通体香采用同一提取方法所得精油化学成分相似,而奇楠沉香中含有更多含量的香味物质和2-(2-苯乙基)色酮类化合物。3种超临界萃取沉香精油清除DPPH自由基能力均强于水蒸气蒸馏沉香精油,而抗炎活性则相反,且3种沉香Objective To compare the compounds composition, antioxidant capacity and anti-inflammatory activity of six kinds of agarwood essential oils from different sources and different extraction methods. Methods The chemical constituents of six agarwood essential oils were analyzed by gas chromatography-time of flight mass spectrometry(GC-TOF MS), their free radical scavenging abilities against 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH) and 2,2-azabis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt(ABTS) were detected, and effect of six agarwood essential oils on lipopolysaccharide(LPS)-induced RAW264.7 cells viabilities were detected. Results The number of compounds identified from BTS(essential oil extracted by hydrodistillation from ordinary agarwood of trunk-prunning), TTS(essential oil extracted by hydrodistillation from ordinary agarwood of Agar-Wit), QNS(essential oil extracted by hydrodistillation from Chi-Nan agarwood of drilling), BTC(agarwood essential oil extracted by supercritical fluid from ordinary agarwood of trunk-prunning), TTC(agarwood essential oil extracted by supercritical fluid from ordinary agarwood of Agar-Wit), QNC(agarwood essential oil extracted by supercritical fluid from Chi-Nan agarwood of drilling) were 48, 55, 59, 71, 72 and 35, respectively;Total contents of aromatic compounds were 81.42%, 87.84%, 89.03%, 62.05%, 63.83% and 28.36%, respectively;Contents of 2-(2-phenylethyl) chromones were 0, 0, 0.25%, 22.42%, 17.48% and 60.42% respectively. Six agarwood essential oils all had the ability to scavenge DPPH and ABTS free radicals, and the essential oils were positively correlated with the free radical scavenging ability between 0.5—5 mg/m L. Half inhibition concentration(IC50) of agarwood essential oils extracted by hydrodistillation(BTS, TTS, QNS) on LPS-induced RAW264.7 cells were 18.61, 30.30, 18.05 μg/m L, and IC50 values of three agarwood essential oils extracted by supercritical fluid(BTC, TTC, QNC) were 41.98, 38.45, 24.50 μg/m L. Conclusion Agarwood essential oils ex

关 键 词：沉香 奇楠 精油 化学成分 抗氧化能力 抗炎活性 2-(2-苯乙基)色酮 

分 类 号：R285.5[医药卫生—中药学]