紫花苜蓿ADK基因家族成员鉴定与分析  

Identification and analysis of ADK gene family members in alfalfa(Medicago sativa)

作　　者：王仪明 雷艳芳 徐俊山 魏臻武[3] 魏佳 闵学阳 WANG Yiming;LEI Yanfang;XU Junshan;WEI Zhenwu;WEI Jia;MIN Xueyang(Dingying Agriculture Co.,Ltd.,Shanghai 202178,China;Bright Food(Group)Co,Ltd.,Shanghai 200040,China;College of Animal Science and Technology,Yangzhou University,Institute of Pratacultural Science,Yangzhou University,Yangzhou 225009,Jiangsu,China)

机构地区：[1]上海鼎赢农业有限公司,上海202178 [2]光明食品(集团)有限公司,上海200040 [3]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《草业科学》2022年第9期1803-1814,共12页Pratacultural Science

基　　金：上海市科技兴农项目(沪农科推字(2020)第1-2号);江苏省高等学校自然科学研究项目(22KJB230011);江苏省博士后科研资助计划项目(2021K197B)。

摘　　要：腺苷酸激酶(ADK)是广泛分布于动植物体内维持细胞能量动态平衡的关键酶,在调控植物生长发育和逆境应答等过程中发挥重要功能。基于紫花苜蓿(Medicago sativa)基因组数据,在全基因组水平对ADK基因进行鉴定,通过生物信息学方法分析了MsADK基因的理化性质、基因结构、染色体定位、顺式作用原件、系统进化关系、蛋白二级和三级结构,以及在不同组织和非生物胁迫下的表达模式。结果表明,紫花苜蓿MsADK家族有10个成员,分布于7条染色体上,3对基因为片段复制;编码区序列长度627~1515 bp,平均长度为768 bp;外显子数目以3~6个居多;蛋白表现为亲水性,亚细胞定位多样,主要位于细胞质;系统进化分析表明MsADK在不同物种间高度保守,与豆科植物亲缘关系更近;顺式作用元件的预测结果表明,MsADK启动子区存在许多与激素、胁迫和光响应有关的反应元件。在不同组织和非生物胁迫下的表达模式表明,MsADK基因在茎中的表达丰度更高;在脱落酸(ABA)、干旱和盐胁迫条件下,除MsADK1、MsADK3和MsADK8上调表达外,其余基因随着胁迫时间的延长均下调表达。但是在低温胁迫下,除MsADK2、MsADK5下调表达外,其他成员均上调表达。研究结果表明,MsADK基因在不同胁迫条件下,存在正向和负向两种调控模式。研究结果将为基于ADK基因遗传改良紫花苜蓿提供候选基因和理论依据。Adenylate Kinase(ADK) is a key enzyme widely distributed in animals and plants that maintains the dynamic balance of cell energy. It plays an important role in growth and stress response. We identified MsADK genes at a genomewide level based on the alfalfa genome data. Bioinformatics methods were used to analyze the physical and chemical properties, gene structure, chromosome location, promoter cis-acting, phylogenetic relationship, and secondary and tertiary structure of MsADK. Furthermore, MsADK gene expression in different tissues and its response to abiotic stress was analyzed. Ten members of the MsADK gene family were identified in alfalfa and distributed on seven chromosomes;three of these pairs were segmental duplication. The coding regions were between 627 and 1 515 bp, with an average length of 768 bp. The number of exons was mostly between three and six. MMsADK protein is hydrophilic, and its subcellular location is diverse, mainly located in the cytoplasm. Phylogenetic analysis showed that MsADK is highly conserved among different species and has a close relationship with legumes. The prediction results of cis-acting elements showed that MsADK promoter regions had many response elements related to hormones, stress, and light. Analysis of MsADK expression pattern in different tissues and under abiotic stress revealed that MsADK expression was higher in stems than in other parts.MsADK1, MsADK3, and MsADK8 were up-regulated under ABA, drought, and salt stress conditions, whereas the remaining MsADK genes were down-regulated. MsADK2 and MsADK5 were down-regulated, while all other MsADK genes were upregulated under low-temperature stress. Results also showed that MsADK has two regulatory patterns(positive or negative)under different stress conditions. These results will provide candidate genes and a theoretical basis for the genetic improvement of alfalfa based on the ADK gene.

关 键 词：ADK基因 生物信息学 基因复制 系统进化 生长发育 表达模式 非生物胁迫 

分 类 号：S541.9[农业科学—作物学]