Expression and activity of heterologous hydroxyisocaproate dehydrogenases in Synechocystis sp.PCC 6803ΔhoxYH  

作　　者：Valentina Jurka Christoph K.Winkler Silvan Poschenrieder Paulo Oliveira Catarina C.Pacheco Eunice A.Ferreira Florian Weissensteiner Piera De Santis Selin Kara Robert Kourist Paula Tamagnini Wolfgang Kroutil 

机构地区：[1]a Institute of Chemistry,University of Graz,NAWI Graz,Heinrichstrasse 28,8010 Graz,Austria [2]i3S–Instituto de Investigação e Inovação em Saúde,Universidade do Porto,4200-135 Porto,Portugal [3]IBMC–Instituto de Biologia Molecular e Celular,Universidade do Porto,4200-135 Porto,Portugal [4]Departamento de Biologia,Faculdade de Ciências,Universidade do Porto,4169-007 Porto,Portugal [5]ICBAS–Instituto de Ciências Biomédicas Abel Salazar,Universidade do Porto,4050-313 Porto,Portugal [6]Aarhus University,Department of Engineering,Biological and Chemical Engineering Section,Biocatalysis and Bioprocessing Group,Gustav Wieds Vej 10,DK 8000 Aarhus,Denmark [7]Institute of Molecular Biotechnology,Graz University of Technology,8010 Graz,Austria [8]Field of Excellence BioHealth,University of Graz,8010 Graz,Austria [9]BioTechMed Graz,8010 Graz,Austria.

出　　处：《Engineering Microbiology》2022年第1期1-7,共7页工程微生物学（英文）

摘　　要：Exploiting light to drive redox reactions is currently a hot topic since light is considered as an environmentally friendly source of energy.Consequently,cyanobacteria,which can use light e.g.,for generating NADPH,are in the focus of research.Previously,it has been shown that various heterologous redox enzymes could be expressed in these microorganisms.Here we demonstrated the successful inducer-free expression of𝛼-keto-acid dehydroge-nases(L-HicDH and D-HicDH)from Lactobacillus confusus DSM 20196 and Lactobacillus paracasei DSM 20008 in Synechocystis sp.PCC 6803ΔhoxYH mutant using replicative plasmids.While the L-HicDH showed poor activity limited by the amount of expressed enzyme,the D-HicDH was applied both in vivo and in vitro,transforming the selected𝛼-keto acids to the corresponding optically pure(R)-𝛼-hydroxy acids(ee>99%)in up to 53%and 90%conversion,respectively.

关 键 词：CYANOBACTERIA BIOCATALYSIS Photobiotransformation Synechocystis sp.PCC 6803 Hydroxyisocaproate dehydrogenase Ketoacid dehydrogenase 

分 类 号：Q93[生物学—微生物学]