上调miR-138-5p对舌鳞状细胞癌细胞增殖及上皮间质转化的影响  

Effect of upregulation of miR-138-5p on the proliferation and epithelial mesenchymal transition of tongue squamous cell carcinoma cells

作　　者：杨晋[1] 张娟 高杰[1] 马素伟 王焱[1] 靳昊[1] YANG Jin;ZHANG Juan;GAO Jie;MA Suwei;WANG Yan;JIN Hao(Second Central Hospital of Baoding,Baoding 072750,China)

机构地区：[1]保定市第二中心医院口腔科,河北保定072750

出　　处：《广东药科大学学报》2022年第5期90-96,共7页Journal of Guangdong Pharmaceutical University

基　　金：河北省保定市科学技术研究与发展指导计划项目(18ZF318)。

摘　　要：目的探讨miR-138-5p对舌鳞状细胞癌(TSCC)Tca-8113细胞增殖、凋亡及上皮间质转化(EMT)的影响。方法qRT-PCR检测永生化的舌上皮细胞株Hacta和舌鳞癌细胞Tca-8113中miR-138-5p和EZH2 mRNA表达水平;生物信息学预测和双荧光素酶验证miR-138-5p和EZH2的靶向关系;将Tca-8113细胞随机分为Tca-8113组、miR-138-5p NC组、miR-138-5p mimics组、si-NC组、si-EZH2组、miR-138-5p mimics+pcDNA NC组、miR-138-5p mimics+pcDNA EZH2组。CCK-8法检测细胞增殖活性;流式细胞术检测细胞凋亡率;Transwell小室法检测细胞迁移和侵袭能力;Western blot法检测细胞EMT相关蛋白β-联蛋白(β-catenin)、上皮钙黏素(E-cadherin)、神经钙黏素(N-cadherin)和波形蛋白(vimentin)表达水平。结果与Hacta细胞相比,Tca-8113细胞miR-138-5p表达水平显著降低,EZH2 mRNA和蛋白表达水平显著升高(P<0.05);miR-138-5p和EZH2存在靶向关系。与Tca-8113和miR-138-5p NC组相比,miR-138-5p mimics组miR-138-5p表达水平、细胞凋亡率及β-catenin和E-cadherin表达水平显著升高,EZH2 mRNA和蛋白表达水平、细胞增殖活性、迁移侵袭能力及N-cadherin和vimentin表达水平显著降低(P<0.05)。干扰EZH2可抑制Tca-8113细胞增殖和EMT进程(P<0.05)。过表达EZH2可逆转上调miR-138-5p对Tca-8113细胞凋亡、迁移、侵袭和EMT进程的影响(P<0.05)。结论上调miR-138-5p可通过靶向抑制EZH2表达,降低TSCC细胞Tca-8113增殖、迁移侵袭及EMT,促进其凋亡。Objective To investigate the effects of miR-138-5p on the proliferation,apoptosis and epithelial mesenchymal transition(EMT)of tongue squamous cell carcinoma(TSCC)Tca-8113 cells.Methods qRT-PCR was used to detect the mRNA expression of miR-138-5p and EZH2 in the immortalized tongue epithelial Hactacells and TSCC Tca-8113 cells.Bioinformatics prediction and double luciferase were used to verify the targeting relationship between miR-138-5p and EZH2.Tca-8113 cells were randomly divided into Tca-8113 group,miR-138-5p NC group,miR-138-5p mimics group,si-NC group,si-EZH2 group,miR-138-5p mimics+pcDNA NC group and miR-138-5p mimics+pcDNA EZH2 group.CCK-8 assay was used to detect cell proliferation.The apoptosis rate was detected by flow cytometry.Cell migration and invasion were detected by Transwell chamber assay.Western blot was used to detect the expression of EMT related proteinsβ-catenin,E-cadherin,N-cadherin and vimentin.Results Compared with Hacta cells,the expression level of miR-138-5p was significantly decreased in Tca-8113 cells,but the expression level of EZH2 mRNA and protein was elevated(P<0.05).There was a targeting relationship between miR-138-5p and EZH2.Compared with Tca-8113 group and miR-138-5p NC group,the expression level of miR-138-5p,apoptosis rate and expression levels ofβ-catenin and E-cadherin were significantly higher in miR-138-5p mimics group,but the expression levels of EZH2 mRNA and protein,the cell proliferation activity,migration and invasion ability and the expression levels of N-cadherin and vimentin were significantly lower(P<0.05).Interference with EZH2 inhibited the proliferation and EMT process of Tca-8113 cells(P<0.05).Overexpression of EZH2 reversed the effects of miR-138-5p upregulation on the apoptosis,migration,invasion and EMT process of Tca-8113 cells(P<0.05).Conclusion Upregulation of miR-138-5p can reduce the proliferation,migration,invasion and EMT of Tca-8113 cells and promote its apoptosis by inhibiting EZH2.

关 键 词：miR-138-5p 组蛋白甲基化转移酶基因 舌鳞状细胞癌 增殖 凋亡 上皮间质转化 

分 类 号：R739.86[医药卫生—肿瘤]