Protein expression changes in cornea after collagen crosslinking  

作　　者：Ziwei Yuan Mengyao Yu Dongguo Li Haixia Zhang Lin Li 

机构地区：[1]Beijing Key Laboratory of Fundamental Research on Biomechanics in Clinical Application,Capital Medical University,Beijing,100069,China [2]School of Biomedical Engineering,Capital Medical University,Beijing,100069,China

出　　处：《Medicine in Novel Technology and Devices》2022年第2期48-54,共7页医学中新技术与新装备（英文）

基　　金：This work was financially supported by the National Natural Science Foundation of China(NSFC)(grant number 31370952,31,470,914).

摘　　要：Riboflavin/UV-mediated corneal collagen cross-linking can increase the mechanical strength of the cornea and prevent or delay corneal expansion and keratoconus progression.We performed quantitative analysis of protein iTRAQ in rabbit eye white matter after cross-linking to explore the changes of protein expression in cornea at different times after cross-linking and to understand the process of corneal stroma remodeling after cross-linking.The screening conditions are fold Change1.2 and P-value<0.05,we identified 713 and 38 differentially expressed proteins in cornea at 1 week and 1 month after cross-linking.There were 16 differentially expressed proteins at two time points after corneal cross-linking.By annotating the functions of these proteins,we identified some proteins that affect the mechanical properties of the cornea,and these proteins are involved in cell growth,oxidative stress response,and signal transduction in the cornea.It has a guiding role in studying the corneal stroma remodeling process after collagen crosslinking.

关 键 词：Corneal collagen crosslinking ITRAQ Differentially expressed protein Stroma remodeling 

分 类 号：R779.6[医药卫生—眼科]