miR-199-5p regulates spermiogenesis at the posttranscriptional level via targeting Tekt1 in allotriploid crucian carp  

作　　者：Shengnan Li Qiubei Wang Lu Huang Siyu Fan Ting Li Yuqing Shu Chun Zhang Yi Zhou Qingfeng Liu Kaikun Luo Min Tao Shaojun Liu 

机构地区：[1]State Key Laboratory of Developmental Biology of Freshwater Fish,College of Life Sciences,Hunan Normal University,Changsha 410081,PR China [2]Guangdong Laboratory for Lingnan Modern Agriculture,Guangzhou 510642,PR China

出　　处：《Journal of Animal Science and Biotechnology》2022年第5期1373-1385,共13页畜牧与生物技术杂志（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant No.31872551);Laboratory of Lingnan Modern Agriculture Project(Grant No.NT2021008);the Natural Science Foundation of Hunan Province for Distinguished Young Scholars(Grant No.2020JJ2022);Hunan Provincial Innovation Foundation for Postgraduate(Grant No.CX20200480);the earmarked fund for China Agriculture Research System(Grant No.CARS-45);the Key Research and Development Program of Hunan Province(Grant No.2020NK2016);111 Project(D20007).

摘　　要：Background:Sperm abnormalities are one of the primary factors leading to male sterility,but their pathogenesis is still unclear.Although miRNAs are suggested to exert important roles in the regulation of spermatogenesis at both transcriptional and posttranscriptional levels,little is currently known regarding the regulation of sperm flagella assembly by microRNAs(miRNAs).The role of miRNAs in the development of sperm abnormalities in sterile triploid fish has not been studied.Results:In this study,we found that miR-199-5p was widely expressed in all detected tissues of different-ploidy crucian carp.As one of the testis-specific candidate markers,Tekt1 was predominantly expressed in the testis.Quantitative real-time PCR(qRT-PCR)analyses showed that the expression trend of miR-199-5p was exactly opposite to that of Tekt1.Through bioinformatics analysis,we identified a putative miR-199-5p binding site in the Tekt1 mRNA.We further identified Tekt1 as a target of miR-199-5p using luciferase reporter assay.Finally,we confirmed that miR-199-5p was necessary for sperm flagellar assembly and spermatogenesis in vivo via intraperitoneal injection of miR-199-5p antagomir or agomir in diploid red crucian carp.Moreover,miR-199-5p gainof-function could lead to spermatids apoptosis and abnormal spermatozoa structure,which is similar to that of allotriploid crucian carp.Conclusions:Our studies suggested that abnormally elevated miR-199-5p inhibited the sperm flagella formation in spermiogenesis by negatively regulating the expression of Tekt1,thereby causing sperm abnormalities of male allotriploid crucian carp.

关 键 词：Allotriploid crucian carp miR-199-5p Sperm abnormalities Sperm flagella SPERMIOGENESIS Tekt1 

分 类 号：S917.4[农业科学—水产科学]