两种方法构建小鼠过敏性接触性皮炎的比较研究  

A comparative study of two methods to construct allergic contact dermatitis in mice

作　　者：朱晓筱 周兰兰[1,2] ZHU Xiao-xiao;ZHOU Lan-lan(Dept of Pharmacology,Anhui Medical University,Hefei 230032,China;Dept of Medical College,Shenzhen Polytechnic,Shenzhen 518055,China)

机构地区：[1]安徽医科大学基础医学院药理教研室,安徽合肥230022 [2]深圳职业技术学院医护学院,广东深圳518055

出　　处：《中国药理学通报》2022年第10期1591-1596,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81870957)。

摘　　要：目的探讨2,4-二硝基氟苯(2,4-dinitrofluorobenzene,DNFB)诱导不同建模周期的过敏性接触性皮炎(allergic contact dermatitis,ACD)模型的差异,为在皮肤瘙痒和炎症的研究中明确和选择更适合的动物模型提供参考和依据。方法DNFB作为致敏剂,0.5%DNFB构建2周ACD模型,敏化5 d后,造模部位间隔1 d给药1次,重复4次;0.15%DNFB构建5周ACD模型,敏化1周后,DNFB涂抹造模部位,每周2次,持续4周。每次涂抹DNFB 24 h后记录行为学视频60 min。造模结束后,ELISA检测血清中Ig-E水平,且造模处皮肤进行组织病理学染色并观察。结果两种建模的ACD小鼠整个建模过程都伴随着皮肤反复接触DNFB后的严重刮擦反应,且抓挠次数明显增多(P<0.01)。组织病理学结果显示,两种造模方法均存在表皮增厚(P<0.01),角化过度和炎症性细胞浸润(P<0.01),且血清中Ig-E水平显著升高。结论DNFB引起的ACD模型非常稳定,两种建模周期均表现出瘙痒、皮肤损伤和免疫炎症反应,而5周模型可能有更典型的症状,且有足够时间观察药物的作用,这为进一步研究瘙痒和炎症相关药物提供实验基础和证据。Aim To explore the differences of 2,4-dinitrofluorobenzene(DNFB)-induced allergic contact dermatitis(ACD)models with different modeling cycles for the study of skin itching and inflammation,so as to provide reference and basis for the identification and selection of a more suitable animal model.Methods DNFB was used as a sensitizer,0.5%DNFB was used to build a 2-week ACD model,and after 5-day sensitization,the modeling site was administered once every other day and repeated four times.0.15%DNFB was used to build a 5-week ACD model,and after one week of treatment,DNFB was applied to the modeling site twice a week for four weeks.Behavioral videos were recorded for 60 minutes after each application of DNFB on the back of the neck for 24 hours.After modeling,Ig-E levels in serum were detected by ELISA,and the skin at the modeling site was stained for histopathology and observed.Results The entire modeling process of both modeled ACD mice was accompanied by severe scratching response after repeated skin exposure to DNFB,and the number of scratching significantly increased(P<0.01).Histopathological results showed epidermal thickening(P<0.01),hyperkeratosis and inflammatory cell infiltration(P<0.01)in both modeling methods,and serum Ig-E levels were significantly elevated(P<0.01).Conclusions The contact dermatitis model caused by DNFB is very stable,showing typical pruritus symptoms,severe dermatitis injury and inflammatory immune response,but the 5-week model may have more typical symptoms and allow enough time to observe the effect of the drug,which provides further experimental basis and evidence for pruritus and inflammation related drug research.

关 键 词：过敏性接触性皮炎 2 4-二硝基氟苯 慢性瘙痒 免疫炎症 肥大细胞 动物模型 

分 类 号：R-332[医药卫生] R322.99R363-332R364.5R758.2