电针内关、公孙穴对功能性消化不良大鼠胃肠动力和心率变异性的影响  

Effects of double acupoints electroacupuncture for gastrointestinal motility and heart rate variability of functional dyspepsia rats

作　　者：展立芬 邢博文 覃思敏 谢云方 江钰 伍萍香 刘未艾[1] 石海斌 ZHAN Lifen;XING Bowen;TAN Simin;XIE Yunfang;JIANG Yu;WU Pingxiang;LIU Weiai;SHI Haibin(Department of Acupuncture and Tuina Rehabilitation,The Second Affiliated Hospital of Hunan University of Chinese Medicine,Changsha,Hunan 410005;Second Department of Orthopedics,Zhangjiajie Hospital of TCM,Zhangjiajie,Hunan 427000)

机构地区：[1]湖南中医药大学第二附属医院针灸推拿康复科,湖南长沙410005 [2]湖南省张家界市中医院骨伤二科,湖南张家界427000

出　　处：《河北中医》2022年第7期1153-1158,共6页Hebei Journal of Traditional Chinese Medicine

基　　金：2020年度湖南省自然科学基金项目(编号:2020JJ4472);湖南省中医药管理局2021年度湖南省中医药科研计划项目(编号:2021026);湖南省中医药管理局2021年度湖南省中医药科研计划项目(编号:D2022063);湖南中医药大学研究生科研创新课题(编号:2020CX47)。

摘　　要：目的观察电针内关、公孙穴对功能性消化不良(FD)大鼠悬尾不动时间、心率变异性(HRV)及胃排空率和小肠推进率的影响,为双穴配伍应用提供一定的实验依据。方法将65只SD大鼠按照随机数字表法分为空白组(10只)和模型储备组(55只),空白组正常饲养,模型储备组采用复合病因造模法制备FD模型,造模成功后取50只大鼠按照随机数字表法分为模型组、内关穴组、公孙穴组、内关+公孙穴组和西药组,每组各10只。空白组、模型组不进行任何干预。内关穴组行束缚捆绑+电针内关穴30 min+0.9%氯化钠注射液2 mL/100 g灌服干预,公孙穴组行束缚捆绑+电针公孙穴30 min+0.9%氯化钠注射液2 mL/100 g灌服干预,内关+公孙穴组行束缚捆绑+电针内关、公孙穴30 min+0.9%氯化钠注射液2 mL/100 g灌服干预,西药组予束缚捆绑30 min+氟哌噻吨美利曲辛片溶液0.95 mg/kg、枸橼酸莫沙必利溶液1.32 mg/kg灌服干预。各组均干预21 d。比较各组大鼠悬尾不动时间,HRV频域指标高频功率(HF)、低频功率(LF)/HF及胃排空率和小肠推进率。结果与空白组相比,模型组大鼠悬尾不动时间明显增加(P<0.05),HF降低(P<0.05),LF/HF比值升高(P<0.05),胃排空率及小肠推进率均降低(P<0.05)。与模型组相比,各治疗组大鼠悬尾不动时间均缩短(P<0.05),HF均升高(P<0.05),LF/HF比值均降低(P<0.05),胃排空率和小肠推进率均明显升高(P<0.05)。与内关穴组相比,公孙穴组悬尾不动时间增加(P<0.05),内关+公孙穴组及西药组悬尾不动时间均缩短(P<0.05),公孙穴组、内关+公孙穴组及西药组HF、胃排空率和小肠推进率均升高(P<0.05),LF/HF比值均降低(P<0.05)。与公孙穴相比,内关+公孙穴组及西药组悬尾不动时间均缩短(P<0.05),HF均升高(P<0.05),公孙穴组LF/HF比值升高(P<0.05),内关+公孙穴组和西药组LF/HF比值均降低(P<0.05),胃排空率及小肠推进率均升高(P<0.05)。与西药组相比,内关+公Objective To observe the effects of electroacupuncture(EA)at Neiguan and Gongsun points on the tail suspension fixed time,heart rate variability(HRV),gastric emptying rate(GER)and intestinal propulsive rate(IPR)in functional dyspepsia(FD)rats,and to provide certain guidance for double acupoints.Methods Totally 65 SD rats were randomly divided into the blank group(n=10)and the reserve group(n=55).FD model was successfully established in 50 rats of reserve group by compound etiology modeling method,and they were randomly divided into model group,Neiguan point group(N group),Gongsun point group(G group),Neiguan+Gongsun point group(N+G group)and Western medicine group(W group),10 rats in each group.On the basis of binding+2 mL/100 g administered with 0.9%sodium chloride injection(SCI),N group additionally EA-PC6(neiguan)for 30 min,G group additionally EA-SP4(Gongsun)for 30 min,N+G group additionally EA-PC6(neiguan)for 30 minand EA-SP4(Gongsun)for 30 min,W group was administered with binding for 30 min and conventional Western medicine(Deanxit solution 0.95 mg/kg and mosapride solution 1.32 mg/kg).All groups were intervened for 21 days,the key observation was the tail suspension fixed time,high-frequency(HF)of HRV,low-frequency(LF)/HF of HRV,and GER and IPR.Results Compared with the blank group,the tail suspension fixed time in the model group was significantly increased(P<0.05),HF was decreased(P<0.05),LF/HF was increased(P<0.05),GER and IPR were significantly decreased(P<0.05).Compared with the model group,the tail suspension fixed time in each treatment group was shortened(P<0.05),HF was increased(P<0.05),LF/HF was decreased(P<0.05),and GER and IPR were significantly increased(P<0.05).Compared with N group,the tail suspension fixed time in G group increased(P<0.05),and which in N+G group and W group was shortened(P<0.05).HF,GER and IPR were all increased in G group,N+G group and W group(P<0.05),LF/HF was increased in G group(P<0.05),and LF/HF was decreased in N+G group and W group(P<0.05).Compared with G group,the

关 键 词：消化不良 内关 公孙 电针 模型 动物 胃排空 迷走神经 

分 类 号：R573.905[医药卫生—消化系统] R245.112[医药卫生—内科学]