外源保幼激素对异色瓢虫卵巢发育及生殖相关基因转录水平的影响  

Effects of exogenous juvenile hormone on the ovarian development and transcription levels of the reprodution-related genes in Harmonia axyridis(Coleoptera:Coccinellidae)

作　　者：韩慧[1] 冯朝阳 张硕 何运转[1] HAN Hui;FENG Zhao-Yang;ZHANG Shuo;HE Yun-Zhuan(College of Plant Protection,Hebei Agricultural University,Baoding,Hebei 071000,China)

机构地区：[1]河北农业大学植物保护学院,河北保定071000

出　　处：《昆虫学报》2022年第9期1090-1097,共8页Acta Entomologica Sinica

基　　金：国家重点研发计划(2017YFD0201004);河北省现代农业产业技术体系(HBCT2018060204)。

摘　　要：【目的】本研究旨在明确外源保幼激素(juvenile hormone,JH)对异色瓢虫Harmonia axyridis成虫卵巢发育以及生殖信号通路中关键基因转录水平的影响。【方法】以萝卜蚜Lipaphis erysimi饲喂的异色瓢虫雌成虫为空白对照组,以点滴同体积丙酮的雌成虫为溶剂对照组,对人工饲料饲喂的羽化后第2天的雌成虫点滴不同剂量(80,120和160 ng/头)JHⅢ1,3,5,7和9 d后,解剖成虫卵巢,拍照并测量卵巢长度及其第一卵室的长度和宽度。利用qPCR分析在最适JHⅢ剂量(120 ng/头)处理后1,5和9 d的异色瓢虫雌成虫生殖信号通路中关键基因JH受体methoprene-tolerant(Met)基因、krüppel homolog 1(Kr-h1)基因、卵黄原蛋白(vitellogenin,Vg)基因(Vg1和Vg2)和卵黄原蛋白受体(vitellogenin receptor,VgR)基因表达水平。【结果】与溶剂对照组相比,点滴80和120 ng/头剂量JHⅢ可促进异色瓢虫成虫卵巢发育,其中120 ng/头剂量JHⅢ处理羽化后第2天雌成虫后7 d卵巢中出现大量卵黄沉积,与萝卜蚜饲喂的空白对照组卵巢发育状态一致,卵巢长度以及第一卵室的长度和宽度均显著高于溶剂对照组的;160 ng/头剂量JHⅢ处理则抑制卵巢发育。qPCR结果表明,120 ng/头剂量JHⅢ处理后5和9 d雌成虫中HaMet,HaKr-h1,HaVg1,HaVg2和HaVgR基因表达量较对照组(丙酮处理组)均增加,其中处理后5 d HaMet,HaVg1和HaVgR基因表达量分别为对照组的2.78,13.14和8.28倍,处理后9 d分别为对照组的2.36,1.85和2.01倍,差异极显著。【结论】120 ng/头JHⅢ可促进异色瓢虫成虫卵巢发育,上调处理后5和9 d时雌成虫HaMet,HaKr-h1,HaVg和HaVgR基因表达量;推测这些关键基因在保幼激素调控异色瓢虫卵巢发育及卵黄形成过程发挥重要作用。【Aim】This study aims to clarify the effects of exogenous juvenile hormone(JH)on the ovarian development and transcriptional levels of the key genes in the reproduction-related signaling pathways of Harmonia axyridis.【Methods】The 2-day-old female adults of H.axyridis fed with artificial diet were exposed to different doses(80,120 and 160 ng/individual)of JHⅢby topical application method for 1,3,5,7 and 9 d(the female adults fed with the turnip aphid,Lipaphis erysimi,were used as the blank control group,and those treated with acetone solution of the same volume by topical application method as the vehicle control group),the ovaries were dissected,and the ovarian length and the length and width of their first egg chamber were photographed and measured.qPCR was used to analyze the expression levels of the key genes including JH receptor methoprene-tolerant(Met)gene,krüppel homolog 1(Kr-h1)gene,vitellogenin genes(Vg1 and Vg2)and vitellogenin receptor(VgR)gene in the reproduction-related signal pathways of the female adults of H.axyridis exposed to the optimum dose(120 ng/individual)of JHⅢfor 1,5,and 9 d.【Results】Compared with the vehicle control group,treatments with 80 and 120 ng/individual of JHⅢby topical application method could promote the ovarian development of H.axyridis adults.After the 2-day-old female adults were exposed to 120 ng/individual of JHⅢfor 7 d,a large amount of yolk deposition appeared in the ovary,which was consistent with the ovarian developmental state in the blank control group fed with the turnip aphid,and the ovarian length and the length and width of the first egg chamber were significantly higher than those in the vehicle control group.However,the treatment with 160 ng/individual of JHⅢinhibited the ovarian development of H.axyridis adults.The qPCR results showed that the expression levels of HaMet,HaKr-h1,HaVg1,HaVg2 and HaVgR in female adults at 5 and 9 d after treatment with 120 ng/individual of JHⅢincreased as compared to the those in the control group(acetone

关 键 词：异色瓢虫 外源保幼激素 卵巢发育 信号通路 关键基因 

分 类 号：Q966[生物学—昆虫学]