Structural characterization and immunostimulatory activity of a water-soluble polysaccharide from abalone(Haliotis discus hannai Ino)muscle  

作　　者：Linfan Shi Gengxin Hao Jun Chen Junling Wang Wuyin Weng 

机构地区：[1]College of Ocean Food and Biological Engineering,Jimei University,Xiamen 361021,China [2]Engineering Research Center of the Modern Technology for Eel Industry,Ministry of Education,Xiamen 361021,China

出　　处：《Food Science and Human Wellness》2023年第2期495-502,共8页食品科学与人类健康（英文）

基　　金：financial support received from the National Key R&D Program of China(2021YFD2100200/2021Y FD2100202);National Natural Science Fund(31571835),Fujian Key Project of Natural Science Foundation(2019J02013);the Opening Project of Fujian Provincial Engineering Technology Research Center of Marine Functional Food(Z820239)。

摘　　要：A water-soluble polysaccharide from abalone muscle(AMPP)was isolated.The contents of carbohydrate,protein,uronic acid,and sulfate in AMPP were 83.5%,0.5%,2.7%,and 2.6%,respectively.High-performance liquid chromatography analysis indicated that AMPP was homogeneous and had an average molecular weight of approximately 3.2 kDa.The main monosaccharides of AMPP were glucose(Glc)and mannose with a molar ratio of 99.7:0.3.The structural characteristics of AMPP were elucidated through methylation analysis,Fourier transform infrared spectroscopy,and nuclear magnetic resonance spectroscopy.The linkages of AMPP consisted of terminal,1,4-linked,1,6-linked,and 1,4,6-linked Glcp with a molar ratio of 3.1:7.2:1.0:2.5.In one repeat unit of the proposed AMPP structure,the backbone chain was composed of eight 1→4 glycosidic bonds and one 1→6 glycosidic bond,with three branch chains linked by 1→6 glycosidic bond.In addition,AMPP was found to possess potent immunostimulatory activity via rising phagocytosis of RAW264.7 cells and promoting secretion of TNF-α.

关 键 词：POLYSACCHARIDE Abalone muscle Structural characterization Immunostimulatory activity Nuclear magnetic resonance spectra 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]