Structure elucidation and in vitro rat intestinal fermentation properties of a novel sulfated glucogalactan from Porphyra haitanensis  

作　　者：Peilin Chen Lu Liu Zirun Cheng Yi Zhang Baodong Zheng Xiaoke Hu Hongliang Zeng 

机构地区：[1]Engineering Research Center of Fujian-Taiwan Special Marine Food Processing and Nutrition,Ministry of Education,Fuzhou 350002,China [2]College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China [3]c Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch,Fujian Agriculture and Forestry University,Fuzhou 350002,China [4]Key Laboratory of Coastal Biology and Bioresource Utilization,Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,China

出　　处：《Food Science and Human Wellness》2023年第2期596-606,共11页食品科学与人类健康（英文）

基　　金：supported by the Scientific Research Foundation of Graduate School of Fujian Agriculture and Forestry University(1122yb065);the Program for Leading Talent in Fujian Provincial University(660160190)。

摘　　要：This study was to investigate the structure and rat fecal microbial fermentation properties of a polysaccharide fraction(PHP2)isolated from the red marine alga Porphyra haitanensis.PHP2 was characterized as a sulfated glucogalactan,with a hypothetical backbone structure of→4)Gα(1→6)G4 Sβ(1→4)Glc(1→and a side chain of Man(1→6)Glc.PHP2 had an irregular spherical chain conformation.The 16 S r RNA sequence analysis revealed that PHP2 modulated the rat fecal micro-flora composition,with a similar effect to inulin,changing the dominant genus(Lactobacillus and Escherichia-Shigella)and promoting the growth of organisms that degrade sulfur-containing polysaccharides,such as Desulfovibrio,Ruminococcaceae_UCG-005,and Ruminococcus_2.PHP2 can promote production of acetic,propionic and butyric acid by rat fecal micro-flora.Prediction of metabolic function suggested that PHP2 could modulate cholesterol metabolism.The sulfated glucogalactan fermentation behavior may be associated with its monosaccharide composition,chain branching and chain conformation.PHP2 appeared to have considerable potential as functional food,and was associated with sulfur-containing polysaccharides in general.

关 键 词：Porphyra haitanensis Structure characterization Fecal microflora Short-chain fatty acids Metabolic function prediction POLYSACCHARIDE 

分 类 号：TS254.58[轻工技术与工程—水产品加工及贮藏工程]