Effect of fructooligosaccharides on the colonization of Lactobacillus rhamnosus AS 1.2466^(T) in the gut of mice  

作　　者：Zhihua Niu Meijuan Zou Tingting Bei Na Zhang Dongyao Li Miaoshu Wang Chen Li Hongtao Tian 

机构地区：[1]College of Food Science and Technology,Hebei Agricultural University,Baoding 071000,China [2]College of Biochemistry and Environmental Engineering,Baoding University,Baoding 071000,China [3]New Hope Tensun(Hebei)Dairy Co.,Ltd,Baoding 071000,China [4]Hebei Technology Innovation Center of Probiotic Functional Dairy Product,Baoding 071000,China [5]National Engineering Research Center for Agriculture in Northern Mountainous Areas,Baoding 071000,China

出　　处：《Food Science and Human Wellness》2023年第2期607-613,共7页食品科学与人类健康（英文）

基　　金：supported by the Science and Technology Research Projects of Colleges and Universities in Hebei province(ZD2021059);the Key Research and Development Program of Hebei Province(19227134D);the Natural Science Foundation of Hebei province(C2016204129,C2017204094);the Food Processing Discipline Group of Hebei Agricultural University(Grant No.2021-08)。

摘　　要：Lactobacillus rhamnosus and fructooligosaccharides(FOS)have been widely studied so far.However,the effects of L.rhamnosus on the intestinal microecological environment at the species level and the effect of different proportions of FOS on L.rhamnosus colonization in different parts of mice intestine are still unclear.The study results indicated that the specific bands of enterobacterial repetitive intergenic consensus polymerase chain reaction(ERIC-PCR)in the L.rhamnosus(LR)group significantly increased at 7 days.Although the number of bands was similar to the natural recovery(NR)group,the brightness of few bands significantly enhanced in the later stage of recovery.Besides,Southern-blot maps showed strong signals,indicating that the ERIC-PCR fingerprint could accurately reflect the changes in the mouse gut microbiota diversity.Further,the high-throughput results confirmed that the Lactobacillus and Akkermansia had different changes at different periods,but all of them showed an upward trend,while the Klebsiella were inhibited,thereby maintaining the intestinal microecology balance.Moreover,FOS exerted a positive effect on L.rhamnosus colonization in the gut.

关 键 词：Intestinal microbiota SYNBIOTICS FRUCTOOLIGOSACCHARIDES Lactobacillus rhamnosus 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]