Structure elucidation,immunomodulatory activity,antitumor activity and its molecular mechanism of a novel polysaccharide from Boletus reticulatus Schaeff  

作　　者：Siyuan Su Xiang Ding Yiling Hou Binbin Liu Zhouhe Du Junfeng Liu 

机构地区：[1]Key Laboratory of Southwest China Wildlife Resources Conservation,College of Life Sciences,China West Normal University,Nanchong 637009,China [2]Sericultural Research Institute,Sichuan Academy of Agricultural Sciences,Nanchong 637000,China [3]College of Environmental Science and Engineering,China West Normal University,Nanchong 637009,China

出　　处：《Food Science and Human Wellness》2023年第2期647-661,共15页食品科学与人类健康（英文）

基　　金：supported by the Open Project Program of Irradiation Preservation Technology Key Laboratory of Sichuan Province,Sichuan Institute of Atomic Energy(FZBC2020009);the Open Research Fund Program of Departmental and Municipal Co-construction of Crops Genetic Improvement of Hill Land Key Laboratory of Sichuan Province(2021CGIHL02);Science and Technology Support Project of Nanchong Science and Technology Bureau of Sichuan Province(20YFZJ0053 and 20YFZJ0054);the Sericulture Innovation Team of Sichuan Province(SCCXTD-2021-17);Laboratory of Sichuan Province(2021CGIHL02)。

摘　　要：A new water-soluble heteropolysaccharide with a molecular weight of 15 k Da was isolated from the fruiting bodies of Boletus reticulatus Schaeff.Structural characterization results revealed that B.reticulatus Schaeff polysaccharide(BRS-X)had a backbone of 1,6-linkedα-D-galactose and 1,2,6-linkedα-D-galactose which branches were mainly composed of a terminal 4-linkedβ-D-glucose and the ratio of D-galactose and D-glucose was 5:1.Bioactivity assays indicated that BRS-X displayed a strong proliferative activity in T cells and B cells and promoted the secretion of immunoglobulin G(Ig G),Ig E,Ig D and Ig M.In addition,BRS-X could facilitate the proliferation and phagocytosis of RAW264.7 cells and could significantly inhibit the growth of tumors in S180-bearing mice.The results of transcriptome sequencing analysis illustrated that total 46 genes enriched in MAPK and total 34 genes enriched in PI3 K/Akt signaling pathways in BRS-X group.The protein VEGF and VEGFR expression were significantly reduced under the treatment with BRS-X.These findings provide a scientific basis for the edible and medicinal value of BRS-X.

关 键 词：Boletus reticulatus Schaeff POLYSACCHARIDES Structure identification Immunomodulatory activity Antitumor activity 

分 类 号：R73[医药卫生—肿瘤]