ITGA2表达增加与胰腺癌的进展和预后不良相关研究  

Association Between Increased ITGA2 Expression in Pancreatic Cancer and Its Progression and Poor Prognostic

作　　者：吕朝旭 史恺 LV Zhaoxu;SHI Kai(Department of Basic Medical Sciences,Panzhihua University,Panzhihua 617000,Sichuan)

机构地区：[1]攀枝花学院基础医学院,四川攀枝花617000

出　　处：《攀枝花学院学报》2022年第5期109-118,共10页Journal of Panzhihua University

基　　金：北方民族大学校级科研项目“晚唐五代至北宋前期文学的演进研究”(2017WSH00)。

摘　　要：整合素α2(integrinα2,ITGA2)的异常表达与多种不同类型的肿瘤有关,包括乳腺癌、肠道肿瘤、结直肠癌、胃癌、肝细胞癌、前列腺癌等,但其作用在不同类型的肿瘤发生发展中存在争议。此外,ITGA2在胰腺癌中的潜在作用仍不清楚。在本研究中,利用来自TGGA、ICGC和GTEx数据库,通过生物信息学分析评估了ITGA2在胰腺癌中的功能。从TCGA、ICGA和GTEx数据库下载基因表达数据和相应的临床信息。采用Wilcoxon秩和检验评估胰腺癌组织与正常胰腺组织之间ITGA2表达的变化。然后采用Kruskal-Wallis检验和Logistic回归两种方法研究ITGA2与传统临床病理特征之间的关系。通过Kaplan-Meier生存、Cox回归分析评估ITGA2在胰腺癌中的预后价值。此外,使用基于JAVA的GSEA软件3.0进行单基因集富集分析(s-GSEA)。与正常胰腺组织相比,胰腺癌组织中ITGA2的表达上调。相关性分析结果表明,胰腺癌中ITGA2表达升高与高级别、分期和癌症状态有明显的相关性。生存分析结果表明,与ITGA2低表达的胰腺癌患者相比,ITGA2高表达的预后较差,总生存时间较短。进一步表明,ITGA2是胰腺癌的独立预后相关因素。此外,s-GSEA显示胰腺癌、子宫内膜癌、前列腺癌、甲状腺癌、膀胱癌、肾细胞癌和紧密连接等11个信号通路在ITGA2高表达表型中显着富集。综上所述,我们的研究表明,ITGA2的表达与胰腺癌的进展和预后不良显着相关,这表明ITGA2是胰腺癌的潜在预后标志物。The aberrant expression of ITGA2 has significant correlation with many different types of human tumor,including breast cancer,intestinal tumors,colorectal cancer,gastric cancer,hepatocellular carcinoma,prostate cancer,however,disputes arise regarding its function in the occurrence and development of different kinds of tumors.In addition,the potential role of ITGA2 in pancreatic cancer remains unclear.In this study,we evaluated the function of ITGA2 in pancreatic cancer by means of bioinformatics analysis on the basis of open data from TGGA,ICGC and GTEx database.Data of gene expression and corresponding clinical information were download from TCGA,ICGA and GTEx database.The variations of ITGA2 expression between pancreatic cancer tissues and normal pancreas tissues was evaluated with Wilcoxon rank sum test.Then the relationship between ITGA2 and traditional clinical clinicopathologic features was investigated with two methods,including Kruskal-Wallis test and logistic regression.The prognostic values of ITGA2 in pancreatic cancer was evaluated through Kaplan-Meier survival,Cox regression analysis.Besides,single-Gene Set Enrichment Analysis(s-GSEA)was conducted by using JAVA based GSEA software 3.0.The expression of ITGA2 was up-regulated in pancreatic cancer tissues compared with the normal pancreas tissues.The results of correlation analysis indicated that increased ITGA2 expression in pancreatic cancer has obvious relevance with high grade,stage and cancer status.The results of survival analysis manifested that high expression of ITGA2 had a poor prognostic and shorter overall survival time compared with ITGA2-low expression patients in pancreatic cancer.Furthermore,it was proved that ITGA2 is an independent prognostic relevant factors in pancreatic cancer.In addition,s-GSEA showed that 11 signaling pathways including pancreatic cancer,endometrial cancer,prostate cancer,thyroid cancer,bladder cancer,renal cell carcinoma and tight junction were significantly enriched in ITGA2 high expression phenotype.In conclus

关 键 词：整合素Α2 胰腺癌 生物信息学分析 预后 基因富集分析 

分 类 号：R735.9[医药卫生—肿瘤]