Mapping a mammalian adult adrenal gland hierarchy across species by microwell-seq  

作　　者：Shujing Lai Lifeng Ma Weigao E Fang Ye Haide Chen Xiaoping Han Guoji Guo 

机构地区：[1]Center for Stem Cell and Regenerative Medicine,Zhejiang University School of Medicine,Hangzhou 310058,China [2]Bone Marrow Transplantation Center,The First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310009,China [3]Institute of Hematology,Zhejiang University,Hangzhou 310058,China

出　　处：《Cell Regeneration》2020年第1期110-121,共12页细胞再生（英文）

基　　金：This work was supported by grants from the National Natural Science Foundation of China(81770188,31722027,and 31701290);the National Key Program on Stem Cell and Translational Research(2017YFA0103401).

摘　　要：Recently,single-cell RNA-seq technologies have been rapidly updated,leading to a revolution in biology.We previously developed Microwell-seq,a cost-effective and high-throughput single cell RNA sequencing(scRNA-seq)method with a very simple device.Most cDNA libraries are sequenced using an expensive Illumina platform.Here,we present the first report showing combined Microwell-seq and BGI MGISEQ2000,a less expensive sequencing platform,to profile the whole transcriptome of 11,883 individual mouse adult adrenal gland cells and identify 18 transcriptionally distinct clusters.Moreover,we performed a single-cell comparative analysis of human and mouse adult adrenal glands to reveal the conserved genetic networks in these mammalian systems.These results provide new insights into the sophisticated adrenal gland hierarchy and provide a benchmark,low-cost strategy for highthroughput single-cell RNA study.

关 键 词：ADRENAL MAPPING seq 

分 类 号：R73[医药卫生—肿瘤]