Activation of the Melanocortin-4 receptor signaling byα-MSH stimulates nervedependent mouse digit regeneration  

作　　者：Hanqian Xu Hailin Zhang Yanqing Fang Huiran Yang Ying Chen Chao Zhang Gufa Lin 

机构地区：[1]Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education,Orthopaedic Department of Tongji Hospital,School of Life Sciences and Technology,Tongji University,Shanghai,China [2]Stem Cell Institute,University of Minnesota,Minneapolis,MN 55455,USA [3]Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine,Shanghai East Hospital,Shanghai Key Laboratory of Signaling and Disease Research,Frontier Science Center for Stem Cell Research,School of Life Sciences and Technology,Tongji University,Shanghai,China

出　　处：《Cell Regeneration》2021年第1期215-225,共11页细胞再生（英文）

基　　金：This work was supported by grants from the National Natural Science Foundation of China(Grant No.31771283&31771608);the National Institute of Health(R01HD084440).

摘　　要：Background:Expression of Mc4r in peripheral organs indicates it has broader roles in organ homeostasis and regeneration.However,the expression and function of Mc4r in the mouse limb and digit has not been fully investigated.Our previous work showed that Mc4r−/−mice fail to regenerate the digit,but whether activation of MC4R signaling could rescue digit regeneration,or stimulate proximal digit regeneration is not clear.Results:We analyzed the expression dynamics of Mc4r in the embryonic and postnatal mouse limb and digit using the Mc4r-gfp mice.We found that Mc4r-GFP is mainly expressed in the limb nerves,and in the limb muscles that are undergoing secondary myogenesis.Expression of Mc4r-GFP in the adult mouse digit is restricted to the nail matrix.We also examined the effect ofα-MSH on mouse digit regeneration.We found that administration ofα-MSH in the Mc4r+/−mice rescue the delayed regeneration of distal digit tip.α-MSH could rescue distal digit regeneration in denervated hindlimbs.In addition,α-MSH could stimulate regeneration of the proximally amputated digit,which is non-regenerative.Conclusions:Mc4r expression in the mouse limb and digit is closely related to nerve tissues,andα-MSH/MC4R signaling has a neurotrophic role in mouse digit tip regeneration.

关 键 词：MC4R Mc4r-gfp Α-MSH Digit regeneration Distal Proximal DENERVATION Neurotrophic function 

分 类 号：S82[农业科学—畜牧学]