长非编码反义RNA FMR1-AS1及其正义RNA FMR1在宫颈癌中的表达  

Expression levels of long non-coding antisense RNA FMR1-AS1 and its sense-strand RNA FMR1 in uterine cervical cancer

作　　者：黄秋霞 欧海玲[1] 黄克强[1] 刘姗姗[1] HUANG Qiuxia;OU Hailing;HUANG Keqiang;LIU Shanshan(Department of Pathology,First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,China)

机构地区：[1]广西中医药大学第一附属医院病理科,南宁530023

出　　处：《临床与病理杂志》2022年第9期2074-2083,共10页Journal of Clinical and Pathological Research

基　　金：广西壮族自治区卫生和计划生育委员会科研课题(Z2016658)。

摘　　要：目的:通过整合计算多中心样本研究长非编码反义RNA FMRP翻译调节因子1反义RNA 1 (FMRP translationalregulator1antisenseRNA1,FMR1-AS1)与相应正义RNAFMR1在宫颈癌中的表达。方法:从癌症基因组图谱(The Cancer Genome Atlas,TCGA)和基因表达综合(Gene Expression Omnibus,GEO)数据库检索并下载宫颈癌相关高通量数据集。箱图和独立样本t检验用于比较宫颈癌组织与非癌宫颈组织中FMR1-AS1、FMR1的表达差异。整合计算标准化均数差(standard mean difference,SMD)用于综合探究FMR1-A S1、FMR1在宫颈癌中的表达水平。使用Pearson相关分析研究FMR1-AS1与FMR1表达的相关性。基因集富集分析(gene set enrichment analysis,GSEA)用于研究相关的信号通路。结果:合并SMD的结果显示FMR1-AS1在宫颈癌组织中高表达(SMD=0.63,95%CI:0.15~1.11)。同时,合并1018例样本(678例宫颈癌组织,340例正常宫颈组织)的结果表明FMR1在宫颈癌组织中的表达显著高于正常宫颈组织(SMD=0.49,95%CI:0.32~0.67)。Pearson相关分析表明:FMR1-AS1与FMR1在宫颈癌组织中的表达呈显著正相关(Pearson’sr=0.4463,P<0.00001)。GSEA结果表明FMR1-AS1和FMR1可能参与基因表达调控相关通路。结论:FMR1-AS1与FMR1在宫颈癌组织中的表达显著上调且两者呈正相关,两者可能共同发挥促癌作用,值得进一步研究二者潜在的调控机制。Objective: To explore the expression level of long non-coding antisense RNA FMRP translational regulator 1 antisense RNA 1(FMR1-AS1) and its relative sense-strand RNA FMR1 in uterine cervical cancer(UCC) through integrated calculation. Methods: UCC-related high-throughput datasets were downloaded from The Cancer Genome Atlas(TCGA) and Gene Expression Omnibus(GEO) databases. Boxplots and Student’s t-test were conducted to compare the differences of FMR1-AS1 and FMR1 expression in UCC and normal cervical tissues. Pooled standard mean difference(SMD) was utilized to investigate the expression level of FMR1-AS1 and FMR1 in UCC. Pearson’s correlation analysis was performed to study the correlation of FMR1-AS1 and FMR1 expression in UCC. Gene set enrichment analysis(GSEA) was used to discuss the relative signaling pathway. Results: The result of pooled study revealed that FMR1-AS1 was overexpressed in UCC(SMD=0.63, 95%CI: 0.15 to 1.11). Meanwhile, the expression of FMR1 in UCC was also higher than that in normal cervical tissues via combination of 1 018 samples(678 cases of UCC and 340 cases of normal cervical tissues;SMD=0.49, 95%CI: 0.32 to 0.67). The result of Pearson’s correlation analysis demonstrated that expression of FMR1-AS1 and FMR1 was positively correlated in UCC. The results of GSEA revealed that FMR1-AS1 and FMR1 may participate the regulation of gene expression pathway. Conclusion: FMR1-AS1 and FMR1 were significantly upregulated in UCC tissues and were positively correlated. FMR1-AS1 and FMR1 may jointly facilitate tumorigenesis in UCC, which deserves further research.

关 键 词：FMR1-AS1 反义RNA 长非编码RNA FMR1 宫颈癌 

分 类 号：R737.33[医药卫生—肿瘤]