四逆汤全方与拆方主要药效成分肠吸收动力学对比研究  

Comparative study on intestinal absorption kinetics of main active components in Sini Decoction and its separated recipes

作　　者：高飞[1] 周菲 甘帅 陈雅兰 傅舒[1] 林美斯 傅超美[1] GAO Fei;ZHOU Fei;GAN Shuai;CHEN Ya-lan;FU Shu;LIN Mei-si;FU Chao-mei(State Key Laboratory of Southwestern Chinese Medicine Resource,School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;School of Acupuncture and Moxibustion,Affiliated Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu 610097,China)

机构地区：[1]西南特色中药资源国家重点实验室,成都中医药大学药学院,四川成都611137 [2]成都中医药大学附属医院针灸学校,四川成都610097

出　　处：《中国中药杂志》2022年第18期5064-5070,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金面上项目(81373987);国家自然科学基金青年基金项目(81603536,81903811);四川省科技厅科技创新人才项目(22CXRC0021,2022JDRC0046);中华中医药学会青年人才托举工程A类项目(2021-QNRC2-A09);国家中医药管理局中医药创新团队及人才支持计划项目(ZYYCXTD-D-202209)。

摘　　要：为了研究四逆汤全方与拆方主要药效成分肠吸收动力学的差异性,进而说明四逆汤全方配伍的科学性和合理性。该研究通过构建大鼠原位肠灌注模型,采用HPLC,测定四逆汤全方或拆方汤液中苯甲酰乌头原碱、苯甲酰次乌头原碱、苯甲酰新乌头原碱、新乌头碱、次乌头碱、甘草酸、甘草苷、6-姜辣素等在十二指肠、空肠、回肠不同肠段的吸收差异。结果显示四逆汤组乌头类生物碱的吸收程度/速度比附子组显示出更好的优势,其中苯甲酰新乌头原碱和次乌头碱在十二指肠、空肠和回肠均为四逆汤组的吸收速度更快和吸收程度更大(P<0.05);甘草酸在四逆汤组十二指肠的吸收程度显著高于甘草组和干姜甘草组(P<0.05),6-姜辣素在四逆汤组回肠段的吸收速度和程度显著高于干姜组(P<0.05)。结果表明干姜与甘草可促进附子中有效成分乌头类生物碱在各肠段的吸收,体现出四逆汤君臣佐使、相须增效的科学内涵。This paper aims to study the difference in the intestinal absorption kinetics of main active components of Sini decoction and its separated recipes and explain the scientificity and rationality of the compatibility of Sini Decoction.A in situ intestinal perfusion rat model was established to evaluate the differences in the absorption of benzoylmesaconine,benzoylaconine,benzoylhypacoitine,mesaconitine,hypaconitine,glycyrrhizic acid,liquiritin and 6-gingerol from Sini Decoction and its separated recipes in the duodenum,jejunum and ileum by high performance liquid chromatography(HPLC).The results indicated that the Sini Decoction group was superior to the Aconiti Lateralis Radix Praeparata group in terms of absorption degree and rate for aconitum alkaloids.The absorption of benzoylmesaconine and hypaconitine in the duodenum,jejunum and ileum was faster and stronger in the Sini Decoction group(P<0.05).The absorption degree of glycyrrhizic acid in the duodenum was significantly higher in the Sini Decoction group than in the Glycyrrhizae Radix et Rhizoma group and the Glycyrrhizae Radix et Rhizoma-Zingiberis Rhizoma group(P<0.05).The absorption rate and degree of 6-gingerol in the ileum in the Sini Decoction group were significantly higher than those in the Zingiberis Rhizoma group(P<0.05).In short,Zingiberis Rhizoma and Glycyrrhizae Radix et Rhizoma can promote the absorption of aconitum alkaloids in different intestinal segments,which reflects the scientific composition of Sini Decoction.

关 键 词：四逆汤 附子 甘草 干姜 全方 拆方 原位肠灌注 

分 类 号：R285.5[医药卫生—中药学]