粒毛盘菌多糖在不同消化阶段的生物活性  

Biological activities of polysaccharides from Lachnum in different simulated digestive stages

作　　者：张鑫淼 何雅玲 叶明[1] ZHANG Xinmiao;HE Yaling;YE Ming(School of Food and Biological Engineering,Hefei University of Technology,Hefei 230009,China)

机构地区：[1]合肥工业大学食品与生物工程学院,合肥230009

出　　处：《生物学杂志》2022年第5期52-57,共6页Journal of Biology

基　　金：国家自然科学基金资助项目(31470146)。

摘　　要：为探究粒毛盘菌多糖在体外模拟消化(唾液、胃液、肠液)过程中的组成成分及生物活性变化,从粒毛盘菌YM40发酵液中提取、纯化得到一种新的胞外多糖(LEP-2a)。采用体外模拟消化模型探究LEP-2a在消化过程中分子质量及化学组成变化;原子力显微镜测定微观形貌;测定LEP-2a在模拟消化不同阶段的抗氧化活性变化及其对消化脂解的影响。结果显示,LEP-2a是分子质量为50.3 ku的杂聚糖,不能被唾液消化,但经胃肠消化后分子质量分别降至47.25 ku和42.36 ku,还原糖含量显著增加,微观高度及分支程度显著降低,表明LEP-2a主要在胃肠消化阶段逐渐降解。此外,LEP-2a经胃肠消化后具有更强的抗氧化活性,并可有效抑制脂质分解。结果为粒毛盘菌多糖体内活性的探究提供基础。In order to explore the changes of components and biological activities of polysaccharides from Lachnum during the in vitro simulated digestion(saliva,gastric and intestinal).A novel extracellular polysaccharide(LEP-2 a)was isolated and purified from Lachnum YM40.The digestive characteristics of LEP-2 a,including the changes of molecular weight(Mw),chemical composition and micromorphology were evaluated by using in vitro simulated digestion model.The antioxidant activities of LEP-2 a in different simulated digestive stages and its effect on lipid digestion were also measured.The results showed that LEP-2 a was a heteroglycan with the Mw of 50.3 ku,which could not be digested by saliva,but the molecular weight decreased to 47.25 ku and 42.36 ku after in vitro simulated gastric and intestinal digestion,respectively.The reducing sugar content increased significantly and the atomic force microscopy showed that the height and branching degree of LEP-2 a decreased significantly after gastrointestinal digestion.The results indicated that LEP-2 a was degraded gradually mainly in gastrointestinal digestive stage.Besides,LEP-2 a had stronger antioxidant activities after gastrointestinal digestion and could inhibit the decomposition of lipids effectively.These results provided a basis for further investigation of the in vivo activities of Lachnum polysaccharides.

关 键 词：粒毛盘菌 多糖 体外模拟消化 抗氧化 消化脂解 

分 类 号：Q946.3[生物学—植物学] Q939.9