大鼠(Rattus norvegicus)微量血培养和染色体标本制作的方法  

A MICROCULTURE METHOD FOR RAT WHOLE BLOOD

作　　者：罗丽华[1] 陈宜峰[1] 单祥年[1] 曹筱梅[1] LUO LI-HUA;CHEN YI-FENG;SHAN XIANG-NIAN;CAO XIAO-MEI(Kunming Institute of Zoology,Academia Sinica)

机构地区：[1]中国科学院昆明动物研究所

出　　处：《Zoological Research》1980年第3期303-311,共9页动物学研究（英文）

摘　　要：本文介绍了大鼠微量血培养和制备染色体标本的简易方法。通过多次反复实验,确证了这个培养方法是有效的和可采用的。在结果讨论中,还比较分析了各种因素(包括培养时间、PHA剂量、pH值和肝素剂量)对细胞生长分裂的影响。同时初步确定了大鼠淋巴细胞分裂周期为16小时左右。This study has introduced a simple microtechnique for obtaining metaphase chromosomes from cardio-blood of living rat.Through repeated experiments,we have confirmed that this culture method is effective and can be used.In the paragraphs of"results"and"discussion",we compared the effect of various factors,including different cultural time,PHA doses,pH values and heparin doses,on cell division and growth.Meanwhile,we have preliminarily determined the division cycle of rat lymphocytes,which is about 16 hours.

关 键 词：微量血 细胞分裂周期 大鼠 染色体标本 细胞生长 制备染色体 肝素剂量 简易方法 

分 类 号：R73[医药卫生—肿瘤]