Metabolomics investigation on antiobesity effects of Corydalis bungeana on high-fat high-sugar diet-induced obese rats  

作　　者：Minghai Fu Terigele Bao Hongzhen Yu LiSha A. HuiFang Li Genna Ba Sungbo Cho 

机构地区：[1]Key Laboratory of Tropical Translational Medicine of Ministry of Education,Hainan Key Laboratory for Research and Development of Tropical Herbs,School of Pharmacy,Hainan Medical University,Haikou 571199,China [2]NMPA Key Laboratory of Quality Control of Traditional Chinese Medicine(Mongolian Medicine),Inner Mongolia Minzu University,Tongliao 028000,China

出　　处：《Chinese Herbal Medicines》2022年第3期414-421,共8页中草药（英文版）

基　　金：supported by China National Natural Science Foundation Project (No.81803845);Inner Mongolia Autonomous Region Natural Science Foundation Project (No.2018MS08040);"Mongolian Medicine Food and Drug Source Protection and Utilization Innovation Team" Construction Project (No.190301);Mongolian Medicine Standardization Research International Cooperation Scienceand Technology Innovation project(No.MDKBZH2018009);Open Project of Key Laboratory of Mongolian Medicine Research and Development project of Ministry of Education (No.MDK2018056)

摘　　要：Objective:Corydalis bungeana(CB)is a well-used medicinal herb in Mongolian folk medicine and has been traditionally applied as an antiobesity agent.However,the evidence-based pharmacological effects of CB and its specific metabolic alterations in the obese model are not entirely understood.This study aimed to utilize untargeted metabolomic techniques to identify biomarkers and gain mechanistic insight into the serum metabolite alterations associated with weight loss and lipid metabolism in obese rats.Methods:A high-fat high-sugar(HFHS)diet was used to induce obese models in rats.CB extract was orally gavaged at 0.18,0.9 and 1.8 g/kg doses for six weeks,and feed intake,body weight,fat pad weight,and blood indexes were measured.Blood serum metabolites were evaluated by gas chromatography/quadrupole time-of-fight tandem mass spectrometry(GC-TOF/MS).Results:The results showed that compared with the obese group,the administration of CB extract caused significant decreases in body weight(P<0.05),feed intake,Lee’s index,and perirenal,mesenteric,epididymal fat weight.CB extract also reduced blood triglyceride and total cholesterol levels(P<0.05)of obese rats.Metabolomic findings showed that nine differential metabolites,including pyruvic acid,D-glucuronic acid,malic acid,dimethylglycine,oxoglutaric acid,pantothenic acid,sorbitol acid,fumaric acid and glucose 6-phosphate were identified under CB treatment and altered metabolic pathways such as TCA cycle,pantothenate and CoA biosynthesis,and glycolysis/gluconeogenesis.Conclusion:This study demonstrated weight loss and lipid lowering effects of CB on HFHS diet-induced obese rats and identified nine metabolites as potential biomarkers for evaluating the favorable therapeutic mechanism of CB via regulation of lipid and glucose metabolism.

关 键 词：Corydalis bungeana Turcz. GC-TOF/MS metabolomics Mongolian medicine OBESITY 

分 类 号：R29[医药卫生—民族医学]