A Preliminary Study on the Evaluation of Human Sperm Head Morphology with a Domestic Digital Holographic Image System  

作　　者：Hong Zhu Yong Zhu Can Sun Feng Jiang 

机构地区：[1]Department of Urology,Obstetrics and Gynecology Hospital,Fudan University,Shanghai 200011,China [2]Human Sperm Bank of Fudan University,Obstetrics and Gynecology Hospital,Fudan University,Shanghai 200011,China [3]Shanghai JiAi Genetics and IVF Institute China-USA Center,Shanghai 200011,China

出　　处：《Phenomics》2022年第2期130-135,共6页表型组学（英文）

基　　金：supported by Shanghai Municipal Science and Technology Major Project(No.:2017SHZDZX01).

摘　　要：The head of sperm was imaged with domestic digital holographic microscopy(DHM),and then the quantitative three-dimensional size information of normal sperm and teratozoospermic sperm was compared and analyzed.DHM sperm imaging and repeated quantitative evaluation were used to determine the morphology of the sperm head in two patients with teratozoospermia and four volunteers with normal semen parameters.Sixty and 139 sperm of teratozoospermia patients and normal people were photographed by digital hologram,respectively.The differences in head height and width were compared and statistically analyzed.The sperm head height of the teratozoospermia group was 3.06±1.66μm,which was significantly lower than that of the normal sperm group(4.54±1.60μm,p<0.01),but there was no significant difference in the head width between the two groups.Compared with the traditional two-dimensional optical microscope observation method,the DHM system can provide three-dimensional quantitative information for the sperm head and thus may help in the comprehensive clinical evaluation of the sperm head structure.

关 键 词：Digital holographic microscopy(DHM) TERATOZOOSPERMIA Human sperm head morphology 

分 类 号：R698[医药卫生—泌尿科学]